The identity of the substrate is proprietary and cannot be disclosed. The kit can work for both GDP1 and GDP2.
Wszystkie zdjęcia(2)
Kluczowe dokumenty
MAK208
GPDH Activity Assay Kit
Sufficient for 100 Colorimetric tests
Synonim(y):
Glycerol 3-Phosphate Dehydrogenase Activity Assay Kit
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352200
NACRES:
NA.84
Polecane produkty
metoda wykrywania
colorimetric
temp. przechowywania
−20°C
informacje o genach
human ... GPD1(2819) , GPD2(2820)
mouse ... GPD1(14555) , GPD2(14571)
rat ... GPD1(60666) , GPD2(25062)
Opis ogólny
Glycerol 3-phosphate dehydrogenase (GPDH; EC 1.1.1.8) catalyzes the reversible conversion of dihydroxyacetone phosphate and NADH to glycerol-3-phosphate and NAD+.[1][2] It forms a glycerol phosphate shuttle and facilitates the transfer of reduced equivalents across the mitochondria to the cytosol.[3] GPDH is important for both carbohydrate and lipid metabolism.[2] Mutations in GPDH gene result in transient infantile hypertriglyceridemia.[1]
Zastosowanie
GPDH (glyceraldhyde-3-phoshate dehydrogenase) activity assay kit has been used to measure the serum and hepatic concentrations of GPDH in rabbit.[4]
Przydatność
Suitable for the detection of Glycerol-3-Phosphate Dehydrogenase Activity in tissue and cell culture samples.
Zasada
The Glycerol 3-Phosphate Dehydrogenase Activity Colorimetric Assay Kit provides a simple and sensitive procedure for measuring GPDH activity in a variety of tissues and cells. GPDH activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of GPDH is the amount of enzyme required to generate 1.0 μmole of NADH per minute at pH 8 at 37 °C.
Ta strona może zawierać tekst przetłumaczony maszynowo.
Tylko elementy zestawu
Numer produktu
Opis
- GPDH Assay Buffer
- GPDH Substrate
- GPDH Probe
- NADH Standard
- GPDH Positive Control
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
Kod klasy składowania
8A - Combustible corrosive hazardous materials
Wybierz jedną z najnowszych wersji:
Certyfikaty analizy (CoA)
Lot/Batch Number
Nie widzisz odpowiedniej wersji?
Jeśli potrzebujesz konkretnej wersji, możesz wyszukać konkretny certyfikat według numeru partii lub serii.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
B. Elmahdi, MA Al-Omair, AA El-Bessoumy and SM El-Bahr.
Thomson I S I
International Journal of Pharmaceutics, 12(4), 429-434 (2016)
A compound heterozygous mutation in GPD1 causes hepatomegaly, steatohepatitis, and hypertriglyceridemia.
Joshi M, et al.
European Journal of Human Genetics, 22(10), 1229-1229 (2014)
Regulation of adenine nucleotide translocase and glycerol 3-phosphate dehydrogenase expression by thyroid hormones in different rat tissues.
DUMMLER K, et al.
The Biochemical Journal, 317(3), 913-918 (1996)
Structure of glycerol-3-phosphate dehydrogenase (GPD1) from Saccharomyces cerevisiae at 2.45 ? resolution.
Alarcon D A, et al.
Acta Crystallographica Section F, Structural Biology and Crystallization Communications, 68(11), 1279-1283 (2012)
-
What is the substrate used for detection in this kit? The description indicates that it can be used for both GPD1 and GPD2 (mitochondrial GPDH) activity detection.
1 answer-
Helpful?
-
-
Which substrate is used for the detection in the activity assay kit for mGPDH (GPD2)
1 answer-
The substrate used for detection in the MAK208 kit for measuring Glycerol 3-phosphate dehydrogenase (GPDH) activity is dihydroxyacetone. This kit specifically measures cytosolic GPDH, identified by the EC number 1.1.1.8, and it quantifies the production of NADH. Cytosolic GPDH catalyzes the conversion of sn-glycerol 3-phosphate and NAD into NADH, glycerone phosphate, and H+. On the other hand, mitochondrial GPDH, identified by the EC number 1.1.5.3, catalyzes a different reaction involving sn-glycerol 3-phosphate and quinone into glycerone phosphate and quinol.
Helpful?
-
Active Filters
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej
