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FG0100

Free Glycerol Determination Kit

1 kit sufficient for 1000 reactions

Synonim(y):

Triglyceride and Free Glycerol Kits and Reagents

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Do Państwa/SKUDostępnośćCena netto
1 kit
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
10 110,00 zł

Informacje o tej pozycji

NACRES:
NA.26
UNSPSC Code:
12352202

10 110,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
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Quality Segment

200

usage

 kit sufficient for 1000 reactions

detection method

colorimetric

storage temp.

2-8°C

General description

Glycerol, also referred to as glycerin or glycerine, is a 3 carbon sugar alcohol that forms the backbone of fatty acids. It is a central component for synthesis of all lipids, and acts as a backbone for triglycerides and phospholipids, which play an important role in cell membrane structure. Due to its low toxicity, glycerol is widely used in pharmaceutical, food and cosmetic formulations and is the main waste by-product of biodiesel production via transesterification. In addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed.

Application

Suitable for the quantitative enzymatic determination of glycerol in serum or plasma.
The Free Glycerol Determination Kit has been used to determine the level of glycerol released into the medium from mammalian cells grown in vitro . [1]
The Free Glycerol Determination Kit measures free, endogenous glycerol using coupled enzyme reactions and does not include initial lipase hydrolysis. Glycerol is phosphorylated by adenosine-5′-triphosphate (ATP) forming glycerol-1-phosphate (G-1-P) and adenosine-5′-diphosphate (ADP) in the reaction catalyzed by glycerol kinase (GK). G-1-P is then oxidized by glycerol phosphate oxidase (GPO) to dihydroxyacetone phosphate (DAP) and hydrogen peroxide (H2O2). Peroxidase catalyzes the coupling of H2O2 with 4-aminoantipyrine (4-AAP) and sodium N-ethyl-N-(3-sulfopropyl) m-anisidine (ESPA) to produce a quinoneimine dye that shows an absorbance maximum at 540 nm. The increase in absorbance at 540 nm is directly proportional to the free glycerol concentration of the sample.
The triglyceride and free glycerol kits are for the quantitative determination of glycerol, total triglycerides or free triglycerides.

Other Notes

In addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed.
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TR0100MAK117CS0005
Free Glycerol Determination Kit 1 kit sufficient for 1000 reactions

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Free Glycerol Determination Kit

Serum Triglyceride Determination Kit 1 kit sufficient for 250 tests

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TR0100

Serum Triglyceride Determination Kit

Glycerol Assay Kit sufficient for 200 colorimetric or fluorometric tests

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MAK117

Glycerol Assay Kit

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Sigma-Aldrich

CS0005

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Quality Level

200

Quality Level

200

Quality Level

-

Quality Level

-

detection method

colorimetric

detection method

-

detection method

colorimetric, fluorometric

detection method

-

usage

 kit sufficient for 1000 reactions

usage

 kit sufficient for 250 tests

usage

sufficient for 200 colorimetric or fluorometric tests

usage

sufficient for 100 or 400 colorimetric or fluorometric tests

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Klasa składowania

10 - Combustible liquids

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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