M3942

DMEM - high glucose

MegaCell^™, with sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell cutlure

• Synonim(y): DMEM, DMEM Supplement
• Kod UNSPSC: 12352207
• NACRES: NA.75

Właściwości

• Nazwa produktu: MegaCell^™ Dulbecco′s Modified Eagle′s Medium, liquid, sterile-filtered, suitable for cell culture
• sterylność: sterile-filtered
• Poziom jakości: 300
• Formularz: liquid
• metody: cell culture | mammalian: suitable
• zanieczyszczenia: endotoxin, tested
• komponenty: L-glutamine: no; glucose: 4500 mg/L (Dextro); sodium pyruvate: yes
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

MegaCell^™ is a multifaceted media developed to reduce the amount of serum required for culturing mammalian cells in vitro. It reduces the supplementation of fetal bovine serum (FBS) or other serum from 10% to 3% and supports proliferative rates and maximal cell densities. MegaCell enables maintaining a long-term culture of non-fastidious cell lines.

Zastosowanie

MegaCell^™ Dulbecco′s Modified Eagle′s Medium has been used:

• as a component of the M10 medium to culture CD133⁺ cells from biopsies of para-spinal and quadriceps muscle of Duchenne muscular dystrophy patients
• as a component of the proliferation medium for the expansion and cryopreservation of human induced pluripotent stem (iPS) cells cultured without feeder cells and human embryonic (ES) cell-derived mesoangioblast-like cells
• to culture the positive hybridomas during the production of mouse anti-chicken CD25 monoclonal antibodies

Rekonstytucja

Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)

Informacje prawne

Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.

MegaCell is a trademark of Promega Corp.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable