M1254

MOPS

≥99.5% (titration), crystalline powder

• Synonim(y): 3-(N-Morpholino)propanesulfonic acid, 4-Morpholinepropanesulfonic acid
• Wzór empiryczny (zapis Hilla): C₇H₁₅NO₄S
• Numer CAS: 1132-61-2
• Masa cząsteczkowa: 209.26
• NACRES: NA.25
• PubChem Substance ID: 24896628
• UNSPSC Code: 12161700
• EC Number: 214-478-5
• MDL number: MFCD00006183
• Beilstein/REAXYS Number: 1106776

Właściwości

• Nazwa produktu: MOPS, ≥99.5% (titration)
• InChI key: DVLFYONBTKHTER-UHFFFAOYSA-N
• InChI: 1S/C7H15NO4S/c9-13(10,11)7-1-2-8-3-5-12-6-4-8/h1-7H2,(H,9,10,11)
• SMILES string: OS(=O)(=O)CCCN1CCOCC1
• assay: ≥99.5% (titration)
• form: crystalline powder
• storage condition: dry at room temperature
• technique(s): affinity chromatography: suitable, electrophoresis: suitable
• color: white
• pH: 2.5-4 (25 °C, 209 g/L)
• Quality Level: 200
• useful pH range: 6.5-7.9
• pKa (25 °C): 7.2
• solubility: water: 0.5 g/mL, clear, colorless
• λ: 33 % in H₂O
• suitability: suitable for HPLC
• application(s): clinical research; diagnostic assay manufacturing; life science and biopharma
• storage temp.: room temp

Opis

General description

MOPS (3-(N-morpholino)propanesulfonic acid) also known as Propane Sultone buffer, is a versatile "Good′s buffer" extensively employed in biological research. It shares structural similarities with MES (2-(N-morpholino)ethanesulfonic acid), featuring a morpholine ring. In terms of physiological relevance, MOPS′ pKa of 7.2 closely aligns with physiological pH, making it suitable for studies requiring a near-neutral environment. Compared to MES with a pKa of 6.1, MOPS offers a more physiologically relevant buffering capacity.

MOPS serves various functions in diverse research domains. As a buffering agent, it can be used to stabilize pH in cell culture media, promoting optimal cell growth and function. Additionally, it may find application in protein purification in chromatography, stabilizes protein solutions, and acts as a reliable running buffer in various electrophoretic techniques, enabling efficient separation and analysis of biomolecules. MOPS also efficiently lyses cells for subsequent protein or nucleic acid extraction and stabilizes enzymes in solution, ensuring their activity for research purposes. It further proves valuable in fine-tuning the pH of growth media for optimal cell growth.

MOPS possesses additional advantages such as minimal interaction with metal ions especially with copper (Cu), nickel (Ni), manganese (Mn), zinc (Zn), cobalt (Co) ions, making it ideal for studies involving metal ions. It exhibits excellent water solubility, facilitating its use in various buffer formulations, and minimal lipid solubility, ensuring impermeability to membranes and minimizing interference with cellular processes. In summary, MOPS is a reliable buffer widely utilized across diverse biological disciplines, distinguished by its versatility, physiological relevance, minimal metal interaction, and ease of use, with its extensive applications in cell biology, protein studies, electrophoresis, and biochemical research.

Application

MOPS has been used as:

• a cell culture additive component in lentiviral particle production
• as a buffering agent in microbial growth medium and nuclei extraction buffer
• as a component of the MMSE-A buffer for isolating heart mitochondria[1]
• as a component of the Synthetic Complete (SC) medium[2]
• as a buffer in fluorescence quenching of NADH[3]

Features and Benefits

• Ideal for Cell Biology and Biochemical research
• Can be used as a Buffer component for Electrophoresis and Protein separation
• Effective Buffering from pH 6.5-7.9 (25 °C) with a pKa of 7.2 (25 °C)
• Highly soluble in water
• Minimal metal ion binding

Packaging

The DRYPOUR^®packaging system for high-quality hygroscopic salts DRYPOUR^®packaging:

• The current DRYPOUR^®packaging configuration is in a 50L nestable drum
• 25KG-DP are the standard prepack offering
• Smaller volumes can be put in the same drum for variable configurations

The benefits of DRYPOUR^®:

• Improves operational efficiency by reducing material preparation time and increasing operator safety
• Minimizes caking of hygroscopic salts
• Offers triple protection: against moisture inside and outside, and against contamination

How does the DRYPOUR^®packaging system Improve your operational efficiency?

• This state-of-the-art design packaging system minimizes caking, thus significantly reducing the time needed for material preparation
• PE drum with our new tamper-evident seal, a polyethylene liner with integrated desiccant bags and a breathable interior Tyvek^®liner
• Desiccant bags are firmly integrated into the polyethylene liner
• Triple protection delivers two invaluable results:

1. Dramatically reduced caking
2. No contamination risk from the desiccant

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

DRYPOUR is a registered trademark of Merck KGaA, Darmstadt, Germany

Tyvek is a registered trademark of E. I. du Pont de Nemours and Company

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• flash_point_f: 230.0 °F - closed cup
• flash_point_c: 110 °C - closed cup
• ppe: dust mask type N95 (US), Eyeshields, Gloves