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L3768

Lysing Enzymes from Aspergillus sp.

solid

Synonim(y):

Cellulase, Lysing Enzymes, Pectinase

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Do Państwa/SKUDostępnośćCena netto
500 mg
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1890,00 zł
1 g
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3400,00 zł

Informacje o tej pozycji

NACRES:
NA.54
UNSPSC Code:
12352204
Biological source:
Aspergillus niger

1890,00 zł


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biological source

Aspergillus niger

Quality Segment

form

solid

technique(s)

cell based assay: suitable

input

plant, fungi and insect sample(s)

suitability

suitable for cell lysis

application(s)

cell analysis

storage temp.

2-8°C

General description

Lysing enzymes are also known as lytic enzymes or cell wall lytic enzymes.

Application

Lysing Enzymes from Aspergillus sp. has been used:

  • to digest the cell wall of Schizosaccharomyces pombe[1]
  • to lyse the mycelium cell wall of Trichoderma harzianum
Lysing Enzymes from Aspergillus sp. has been used to digest the cell walls to create pulse-field gel plugs.

Biochem/physiol Actions

Lysing enzymes are a group of enzymes that can break down the cell walls of microorganisms. These enzymes play a crucial role in various biological processes, including cell lysis, tissue dissociation, and protein extraction. Lysing enzymes are commonly used in research, biotechnology, and clinical applications. They are especially useful in DNA extraction, where they help to release genetic material from cells by breaking down the cell walls. This enables efficient isolation and purification of DNA for downstream analyses. Cellulases are a class of enzymes that degrade cellulose, a major component of plant cell walls, into simpler sugars. These sugars can be utilized as raw materials to produce biofuels, biobased chemicals, plastics, and various other materials. Pectinases are a group of enzymes that hydrolyze pectic substances found in microorganisms and higher plants. They can break down the pectin-containing compounds synthesized by plants and microbes.

Analysis Note

Contains pectinase and cellulase activities.
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Ta pozycja
492916230186247
description

solid

description

powder, ≥750 U/g

description

powder (fine), ~200 U/g

description

powder, slightly beige, ~100 U/mg

technique(s)

cell based assay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

biological source

Aspergillus niger

biological source

-

biological source

Aspergillus niger

biological source

Aspergillus sp. (A. oryzae)

application(s)

cell analysis

application(s)

-

application(s)

-

application(s)

-

suitability

suitable for cell lysis

suitability

-

suitability

-

suitability

-

form

solid

form

powder

form

powder (fine)

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C


Klasa składowania

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

  1. What is the working condition and concentration of this enzyme, especially for filamentous fungi cell wall digestion

    1 answer
    1. The working conditions and concentration of this enzyme is not tested. This process will have to be validated by the end-user It may be helpful to view the peer-reviewed articles:

      https://link.springer.com/article/10.1007/BF00437577
      https://www.sciencedirect.com/science/article/pii/S1087184505001052
      https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2958.2005.04827.x

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