L2524
Lyticase from Arthrobacter luteus
lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret
Synonim(y):
(1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase, Bacterial lyticase, Lysing enzyme
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About This Item
Polecane produkty
pochodzenie biologiczne
bacterial (Arthrobacter luteus)
Poziom jakości
Postać
lyophilized powder
aktywność właściwa
≥2,000 units/mg protein
skład
Protein, ≥20% biuret
metody
cell based assay: suitable
przydatność
suitable for cell lysis
Zastosowanie
diagnostic assay manufacturing
temp. przechowywania
−20°C
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Zastosowanie
Lyticase from Arthrobacter luteus has been used to lyse the fungal cell wall for DNA isolation.
Lyticase from Arthrobacter luteus has been used:
- for spheroplasting the cells
- as a component of digestion solution to incubate yeast cells for digestion of the cell wall
- in the enzymatic hydrolysis of the mycelium precipitate to prepare protoplasts
Działania biochem./fizjol.
Lyticase enzyme is frequently used in fungal research, particularly for species identification using polymerase chain reaction (PCR)-based techniques. It can break down β (1→3) and β (1→4) bonds between glucose units. Lysozyme serves as an indicator of macrophage-mediated host response, correlates with white cell death, and exhibits a high turnover rate. Elevated levels of serum lysozyme have been observed in various chronic inflammatory conditions, inflammatory bowel diseases, hematological disorders, and renal disorders. The c-type lysozyme from hen egg white is commonly used as a model for studying protein structure and function. Muramidase primarily exhibits bactericidal activity against Gram-positive bacteria. The method described in the bulletin for determining molecular masses using gel filtration chromatography is a modified version of existing published techniques. The protein standards included in this kit may be compatible with other chromatographic systems like high-performance liquid chromatography (HPLC). However, certain buffer systems may affect the elution volumes of albumin and carbonic anhydrase. The proteins in this kit have a molecular mass range spanning from 29 kDa to 699 kDa.
Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase to induce partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest the cell walls of yeast and generate spheroplasts from fungi for transformation. It contains β-(1→3)-glucan laminaripentaohydrolase along with β-(1→3)-glucanase, protease, and mannanase activities. Lyticase is used for yeast cells like Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species.
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.
Definicja jednostki
One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.
Postać fizyczna
Partially purified, lyophilized powder containing potassium phosphate buffer salts and stabilizers
Inne uwagi
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
This page may contain text that has been machine translated.
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Resp. Sens. 1
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
Certyfikaty analizy (CoA)
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Protokoły
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
Procedura ta może być stosowana do oznaczania aktywności litykazy przy użyciu drożdży Bakera jako substratu.
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