FLAGIPT1
FLAG® Immunoprecipitation Kit
Synonim(y):
Anti-ddddk, Anti-dykddddk
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352200
NACRES:
NA.32
Polecane produkty
zastosowanie
kit sufficient for 50 reactions
Poziom jakości
klasy chemiczne analitów
proteins (FLAG® Affinity Gels)
metody
immunoprecipitation (IP): suitable
Warunki transportu
wet ice
temp. przechowywania
−20°C
Opis ogólny
Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.
Zastosowanie
Learn more product details in our FLAG® application portal.
Cechy i korzyści
- Utilizes highly specific ANTI-FLAG M2 affinity gel
- No preliminary steps or calibrations
- 3X FLAG™ peptide provides efficient and gentle elution through direct competition
Inne uwagi
All prodedures to be performed at 2-8°C.
Informacje prawne
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Aquatic Chronic 3 - Eye Irrit. 2
Kod klasy składowania
10 - Combustible liquids
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