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F0556

Sigma-Aldrich

Human Fibronectin

from human fibroblasts, liquid, ~0.5 mg/mL, suitable for cell culture

Synonim(y):

Fibronectin

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About This Item

Numer WE:
289-149-2
Numer MDL:
MFCD00131062
Kod UNSPSC:
12352202
eCl@ss:
42020141
NACRES:
NA.75

product name

Fibronectin solution human fibroblasts, cell culture derived, ~0.5 mg/mL, sterile-filtered, BioReagent, suitable for cell culture

pochodzenie biologiczne

human fibroblasts

Poziom jakości

200

sterylność

sterile-filtered

linia produktu

BioReagent

Postać

solution

masa cząsteczkowa

31 kDa

opakowanie

pkg of 100 μL

stężenie

~0.5 mg/mL

metody

cell culture | mammalian: suitable

zanieczyszczenia

HBSAG, none detected
 HCV, none detected
 HIV-1/HIV-2, none detected

numer dostępu NCBI

NM_212482.4

numer dostępu UniProt

P02751

Specyficzność wiązania

Peptide Source: Collagen

Warunki transportu

dry ice

temp. przechowywania

−20°C

informacje o genach

human ... FN1(2335)

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Opis ogólny

Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35.
The matrix protein fibronectin consists of three units that are named Fn1, Fn2 and Fn3. The plasma protein has a molar mass of 31kDa. It is present in loose connective tissue, basement membranes, and the surface of various cell types.

Zastosowanie

Fibronectin solution from human fibroblasts is used for the following applications:
  • It is used in purification of human extracellular matrix (ECM)
  • Coating of tissue culture plates with extracellular matrix components
  • Used during cell culture (For xeno-free, feeder-free culture the cells were passaged in to human fibronectin along with other components)

Fibronectin from human plasma can be used with epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Działania biochem./fizjol.

Fibronectin is a high molecular mass extracellular matrix glycoprotein involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, cell migration/adhesion, and embryogenesis and development. It is used in vitro as a substrate to enhance adherence and proliferation of many cell types. It can bind to macromolecules such as collagen, fibrin, glycosaminoglycans, and Clq, apart from some bacteria.It serves as a critical factor in promoting cell survival and preventing programmed cell death in these cellular contexts. Fibronectin plays a significant role in the wound healing process, and in extracellular matrix (ECM) assembly hence is suitable for tissue engineering applications.

Komponenty

Fibronectin is an adhesive glycoproteins found in both the cell surface and in plasma.

Przestroga

The solution should be stored at -20°C. Under these conditions, the product is stable for at least 2 years. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.

Uwaga dotycząca przygotowania

This product is produced by human fibroblasts and then purified biochemically. It is supplied as a sterile solution at 0.5 mg/mL in a CAPS saline buffer. Before use, the product should be thawed slowly at 2-8°C, forming a clear solution. Avoid any vortexing or vigorous shaking of the solution, which will cause any fibronectin to “crash” out of solution. To coat plates, dilute this product in sterile HBSS solutions and coat with a minimal volume. Then, incubate at 37°C for 1-2 hours and wash 3 times with sterile HBSS and plate cells.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

J R Potts et al.
Current opinion in cell biology, 6(5), 648-655 (1994-10-01)
Significant progress has been made recently in the determination of the structure and assembly of the important matrix protein fibronectin, a molecule mainly constructed from three modular units denoted Fn1, Fn2 and Fn3. Atomic resolution structures are now available for
Sajjad Ahmad et al.
Stem cells (Dayton, Ohio), 25(5), 1145-1155 (2007-01-27)
Human embryonic stem cells (hESCs) are pluripotent cells capable of differentiating into any cell type of the body. It has long been known that the adult stem cell niche is vital for the maintenance of adult stem cells. The cornea
Veronica M Coceres et al.
Frontiers in cellular and infection microbiology, 11, 757185-757185 (2021-12-04)
Trichomonas vaginalis and Tritrichomonas foetus are extracellular flagellated parasites that inhabit humans and other mammals, respectively. In addition to motility, flagella act in a variety of biological processes in different cell types, and extra-axonemal structures (EASs) have been described as
Nathalie Chênais et al.
Scientific reports, 9(1), 2861-2861 (2019-03-01)
Reprogramming of cultured cells using Xenopus egg extract involves controlling four major steps: plasma membrane permeabilization, egg factors import into the nucleus, membrane resealing, and cell proliferation. Using propidium iodide to assess plasma membrane permeability, we established that 90% of
Patricia A Soucy et al.
Matrix biology : journal of the International Society for Matrix Biology, 28(5), 273-283 (2009-04-21)
Extracellular matrix plays a critical role in cellular development by providing signaling cues that direct morphogenesis. In order to study both the cues that natural matrix provides and endothelial cell responses to that information, human fetal lung fibroblasts were used
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