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Merck

D8662

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

Dulbecco’s Phosphate Buffered Saline
2 of 3 reviewers received a sample product or took part in a promotion

Liquid, with MgCl₂ and CaCl₂, sterile-filtered, suitable for cell culture

Synonim(y):

DPBS

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100 ML
66,90 zł
500 ML
140,00 zł
1 L
298,00 zł

66,90 zł


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100 ML
66,90 zł
500 ML
140,00 zł
1 L
298,00 zł

About This Item

Numer MDL:
Kod UNSPSC:
12352207
eCl@ss:
32129211
NACRES:
NA.75

66,90 zł


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Poproś o zamówienie zbiorcze

Nazwa produktu

Dulbecco′s Phosphate Buffered Saline, With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture

Poziom jakości

sterylność

sterile-filtered

Formularz

liquid

metody

cell culture | mammalian: suitable

zanieczyszczenia

endotoxin, tested

komponenty

sodium pyruvate: no
glucose: no (Dextro)

Warunki transportu

ambient

temp. przechowywania

2-8°C

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Opis ogólny

Dulbecco′s Phosphate Buffered Saline (D-PBS) is a balanced salt solution developed for use in tissue culture.

Zastosowanie

D-PBS is a general use isotonic saline solution used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
  • as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
  • to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
  • to wash cells for protein isolation

Dulbecco′s Phosphate Buffered Saline has been used:
  • as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR[1]
  • to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells[2]
  • to wash cells for protein isolation[2]

Działania biochem./fizjol.

Dulbecco′s Phosphate Buffered Saline (D-PBS) exhibits four chief functions:
  • provides cells with water and certain bulk inorganic ions essential for normal cell metabolism
  • serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance
  • when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism
  • provides a buffering system to maintain the medium within the physiological pH range

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produkt powiązany

Numer produktu
Opis
Cennik

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

nwg

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Anna Eskilsson et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(19), 5035-5044 (2017-04-26)
Fever occurs upon binding of prostaglandin E
E Valas Teuma et al.
Journal of cataract and refractive surgery, 45(8), 1177-1182 (2019-07-06)
To compare the capsulotomy rim strength with capsular marks (CMs) to the rim strength without CMs in porcine eyes, and to demonstrate the practicality of CMs for intraoperative toric intraocular lens (IOL) alignment. LENSAR facility, Orlando, Florida, USA. Laboratory study.
L Parmegiani et al.
Journal of assisted reproduction and genetics, 35(10), 1887-1895 (2018-08-04)
To study the efficacy and efficiency of a "universal warming protocol" for vitrified human embryos, based on subsequent steps with 1 and 0.5 M concentration of extracellular cryoprotectant (ECCP). Two studies on patients undergoing fertility treatments via ICSI: a prospective randomized
Ivan Kopljar et al.
British journal of pharmacology, 174(21), 3766-3779 (2017-01-18)
In the pharmaceutical industry risk assessments of chronic cardiac safety liabilities are mostly performed during late stages of preclinical drug development using in vivo animal models. Here, we explored the potential of human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs) to
Kimberly R Long et al.
Molecular biology of the cell, 28(19), 2508-2517 (2017-07-20)
Cells lining the proximal tubule (PT) have unique membrane specializations that are required to maintain the high-capacity ion transport and endocytic functions of this nephron segment. PT cells in vivo acutely regulate ion transport in response to changes in glomerular

Protokoły

Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.

Inkorporacja DNA do komórek eukariotycznych przy użyciu fosforanu wapnia jest powszechną metodą transfekcji. Ten protokół transfekcji może być zoptymalizowany dla wielu różnych typów komórek.

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

Questions

1–2 of 2 Questions  
  1. What is the concentration of MgCl2 and CaCl2?

    1 answer
    1. This formulation contains 100 mg/L of Magnesium Chloride Heptahydrate and 133 mg/L of Calcium Chloride Dihydrate. Please see the link below to review the full formulation of this and other Dulbecco's buffer products:
      https://www.sigmaaldrich.com/product/sigma/d8662#product-documentation

      Helpful?

  2. ¿Disponen de algún estudio de estabilidad de este producto a temperatura Ambiente? (15 - 30 ºC)

    1 answer
    1. Any stability study information is considered proprietary and may not be shared. However, this sterile DPBS solution is quite stable. The product is assigned an expiration date of approximately 2 years from the date of quality release. The item ships at ambient temperature and is stable for up to two weeks to allow for transit time. For long term storage, refrigeration at 2-8°C is recommended.

      Helpful?

Reviews

2 of 3 reviewers received a sample product or took part in a promotion

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  1. 3 Ratings-Only Reviews

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