This formulation contains 100 mg/L of Magnesium Chloride Heptahydrate and 133 mg/L of Calcium Chloride Dihydrate. Please see the link below to review the full formulation of this and other Dulbecco's buffer products:
https://www.sigmaaldrich.com/product/sigma/d8662#product-documentation
D8662
Dulbecco’s Phosphate Buffered Saline
Liquid, with MgCl₂ and CaCl₂, sterile-filtered, suitable for cell culture
Dulbecco’s Phosphate Buffered Saline
2 of 3 reviewers received a sample product or took part in a promotion
Synonim(y):
DPBS
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
100 ML
69,60 zł
500 ML
145,00 zł
1 L
310,00 zł
69,60 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Informacje o tej pozycji
Przejdź do
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócNazwa produktu
Dulbecco′s Phosphate Buffered Saline, With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
sodium pyruvate: no
glucose: no (Dextro)
shipped in
ambient
storage temp.
2-8°C
Quality Level
Szukasz podobnych produktów? Odwiedź Przewodnik dotyczący porównywania produktów
1 of 4
Ta pozycja | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 200 |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. 2-8°C | storage temp. - | storage temp. - | storage temp. 2-8°C |
| impurities endotoxin, tested | impurities endotoxin, tested | impurities - | impurities endotoxin, tested |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility 0.1 μm filtered |
General description
Dulbecco′s Phosphate Buffered Saline (D-PBS) is a balanced salt solution developed for use in tissue culture.
Application
D-PBS is a general use isotonic saline solution used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
- as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
- to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
- to wash cells for protein isolation
Dulbecco′s Phosphate Buffered Saline has been used:
- as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR[1]
- to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells[2]
- to wash cells for protein isolation[2]
Biochem/physiol Actions
Dulbecco′s Phosphate Buffered Saline (D-PBS) exhibits four chief functions:
- provides cells with water and certain bulk inorganic ions essential for normal cell metabolism
- serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance
- when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism
- provides a buffering system to maintain the medium within the physiological pH range
Ta strona może zawierać tekst przetłumaczony maszynowo.
related product
Numer produktu
Opis
Cennik
Klasa składowania
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
L Parmegiani et al.
Journal of assisted reproduction and genetics, 35(10), 1887-1895 (2018-08-04)
To study the efficacy and efficiency of a "universal warming protocol" for vitrified human embryos, based on subsequent steps with 1 and 0.5 M concentration of extracellular cryoprotectant (ECCP). Two studies on patients undergoing fertility treatments via ICSI: a prospective randomized
E Valas Teuma et al.
Journal of cataract and refractive surgery, 45(8), 1177-1182 (2019-07-06)
To compare the capsulotomy rim strength with capsular marks (CMs) to the rim strength without CMs in porcine eyes, and to demonstrate the practicality of CMs for intraoperative toric intraocular lens (IOL) alignment. LENSAR facility, Orlando, Florida, USA. Laboratory study.
Anna Eskilsson et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(19), 5035-5044 (2017-04-26)
Fever occurs upon binding of prostaglandin E
Michele Russo et al.
European journal of heart failure, 21(4), 519-528 (2019-03-08)
Butyric acid (BUT), a short chain fatty acid produced daily by the gut microbiota, has proven beneficial in models of cardiovascular diseases. With advancements in cancer survival, an increasing number of patients are at risk of anticancer drug cardiotoxicity. Here
Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway.
Kimberly R Long et al.
Molecular biology of the cell, 28(19), 2508-2517 (2017-07-20)
Cells lining the proximal tubule (PT) have unique membrane specializations that are required to maintain the high-capacity ion transport and endocytic functions of this nephron segment. PT cells in vivo acutely regulate ion transport in response to changes in glomerular
Protokoły
Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.
Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.
Inkorporacja DNA do komórek eukariotycznych przy użyciu fosforanu wapnia jest powszechną metodą transfekcji. Ten protokół transfekcji może być zoptymalizowany dla wielu różnych typów komórek.
-
What is the concentration of MgCl2 and CaCl2?
1 answer-
Helpful?
-
-
Can this solution be autoclaved?
1 answer-
This solution is already sterile-filtered and can be autoclaved at 121°C, 15 psi for 20 minutes.
Helpful?
-
-
Could you give me the precise composition of this DPBS buffer solution (mixture of different salts)
1 answer-
The composition of this product, Dulbecco's Phosphate Buffered Saline, is available in the following Product Information Sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/137/207/d8662for.pdf
Helpful?
-
-
¿Disponen de algún estudio de estabilidad de este producto a temperatura Ambiente? (15 - 30 ºC)
1 answer-
Any stability study information is considered proprietary and may not be shared. However, this sterile DPBS solution is quite stable. The product is assigned an expiration date of approximately 2 years from the date of quality release. The item ships at ambient temperature and is stable for up to two weeks to allow for transit time. For long term storage, refrigeration at 2-8°C is recommended.
Helpful?
-
Active Filters
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej