CRE BAG2

95082219, mouse unknown/unspecified, Fibroblast

• Kod UNSPSC: 41106514

Właściwości

• Nazwa produktu: CRE BAG2, 95082219
• pochodzenie biologiczne: mouse unknown/unspecified
• tryb wzrostu: Adherent
• kariotyp: Not specified
• morfologia: Fibroblast
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Mouse NIH 3T3 fibroblast, Moloney murine leukaemia virus transfected

Opis linii komórkowej

CRE BAG2 has been established from the NIH 3T3 fibroblast cell line. Cells were transfected with Moloney murine leukaemia virus-derived proviral genomes carrying complementary mutations in the gag-pol or env regions. CRE BAG2 produces BAG ( a β-gal-transducing vector) and can be used for the package of vectors derived from murine leukaemia viruses. The production of recombinant replication-competent helper viruses has never been reported.

Zastosowanie

Virus-packaging assays, cell-lineage marking systems

pożywka hodowlana

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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