HT29 Cell Line human

91072201, human colon, Epithelial

• Synonim(y): HT29 Cells
• Kod UNSPSC: 41106514

Właściwości

• product name: HT29 Cell Line human, 91072201, Caucasian colon adenocarcinoma grade II
• pochodzenie biologiczne: human colon
• tryb wzrostu: Adherent
• kariotyp: 2n = 46, hypertriploid
• morfologia: Epithelial
• produkty: Secretory component of Immunoglobulin A (IgA), Carcinoembryonic antigen (CEA)
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Caucasian colon adenocarcinoma

Opis linii komórkowej

Isolated from a primary tumour in a 44 year old Caucasian female. Forms a well-differentiated adenocarcinoma consistent with colony primary, grade I. Tumours also form in steroid treated hamsters. Has the following HLA profile A1,3; B12,17; Cw5.

Zastosowanie

HT29 Cell Line has been used to determine viral titres of human parechovirus. It has also been used to study the ability of Bifidobacterium and Lactobacillus strains to counteract the toxic effect of C. difficile LMG21717.

Tumourigenicity studies

Profil DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19

pożywka hodowlana

McCoy′s 5A + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% Trypsin; 5% CO₂; 37°C.

Inne uwagi

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