SVCT

94122105, human breast, Epithelial

• Synonim(y): SCTV, SCVT
• Kod UNSPSC: 41106514

Właściwości

• product name: SVCT, 94122105, from human breast
• pochodzenie biologiczne: human breast
• tryb wzrostu: Adherent
• kariotyp: Not specified
• morfologia: Epithelial
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice

Opis

Pochodzenie linii komórkowej

Human breast epithelium, SV40 transformed

Opis linii komórkowej

SVCT has been established from human breast epithelial cells being transfected with SV40. The cell line shows an abnormal karyotype and was not tumourigenic in athymic male nude mice. SVCT have been found positive for the expression of keratin 7, 8 and 18 but negative for keratin 19. Epithelial cells, dome formation when confluent.

Profil DNA

Not specified

pożywka hodowlana

DMEM + 2 mM Glutamine + 5μg/ml Hydrocortisone + 10μg/ml Insulin + 10% Fetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:2 to 1:4, i.e., seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Inne uwagi

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Oświadczenie o zrzeczeniu się odpowiedzialności

This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.

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