K-BALB (K-234)

89092101, mouse embryo, Fibroblast

• Kod UNSPSC: 41106514

Właściwości

• product name: K-BALB (K-234), 89092101
• pochodzenie biologiczne: mouse embryo
• tryb wzrostu: Adherent
• kariotyp: 2n=40
• morfologia: Fibroblast
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Mouse A31 embryo Kirsten sarcoma, transformed

Opis linii komórkowej

Cell morphology is different from the parent clone (A31) and there is a loss of sensitivity to contact inhibition of cell division. The cells are highly tumorigenic. Superinfection with MuLV results in rescue of MSV.

pożywka hodowlana

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.1% trypsin for 3-5 minutes; 5% CO₂; 37°C

Inne uwagi

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