A431 Cell Line human

85090402, human skin, Epithelial

• Synonim(y): A-431 Cells, A431/P Cells
• Kod UNSPSC: 41106514

Właściwości

• Nazwa produktu: A431 Cell Line human, squamous carcinoma, 85090402
• pochodzenie biologiczne: human skin
• tryb wzrostu: Adherent
• kariotyp: Hypertriploid
• morfologia: Epithelial
• produkty: Not specified
• receptory: Epidermal growth factor (EGF)
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human squamous carcinoma

Opis linii komórkowej

Derived from an epidermal carcinoma of the vulva taken from an 85 yr old female. The cells carry large numbers of EGF binding sites and is an indicator cell for anti-TGF binding.

Zastosowanie

A431 Cell Line human has been used:

• to demonstrate that p38 mitogen-activated protein kinase enhances epidermal growth factor-induced apoptosis in A431 cells
• to study melanoma-specific CD44 (a tumor marker and transmembrane receptor) alternative splicing pattern in A431 cells
• to study the anti-cancer activity of antioxidant-rich Cameroonian medicinal plants

Cell cycle studies

pożywka hodowlana

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Ta strona może zawierać tekst przetłumaczony maszynowo.