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293T cell line human

293T cell line human

NOTE: Both the cell line and DNA from the cell line may beavailable for this product. Please choose -1VL or VIAL for cells, or -DNA-5UGfor DNA, 12022001, human kidney, fetal. Small slightly rounded cells, dispersed growth

Synonim(y):

Komórki 293tsA1609neo, Komórki HEK-293-T, Komórki HEK-293T, Komórki HEK293T, Ludzkie embrionalne komórki nerkowe 293T

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About This Item

Kod UNSPSC:
41106514
Informacje o cenach i dostępności nie są obecnie dostępne.

pochodzenie biologiczne

human kidney (fetal)

opis

In Australia, this cell line is only supplied as live culture in flask due to the challenging recovery. Please store at 37°C upon receipt.
Shipping condition: Ambient (Australia)

opakowanie

tube of 5 μg 12022001-DNA-5UG
pkg of vial of cells 12022001-1VL

tryb wzrostu

adherent

morfologia

small slightly rounded cells growing in a dispersed manner

produkty

SV40 T antigen

Warunki transportu

dry ice

temp. przechowywania

−196°C

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Opis ogólny

293T cell line is a stable clone derivative of the human embryonic kidney (HEK) 293 cell line.[1] It expresses the large T antigen of simian virus 40 (SV40).[2]

Opis linii komórkowej

The 293T cell line was created in the laboratory of Michele Calos by transfection of a sub-line of adenovirus-immortalized human embryonic kidney cells with a gene encoding the SV40T-antigen and a neomycin resistance gene. The cell line is competent for replication of vectors carrying the SV40 origin of replication. The line also has favourable tissue culture, transfection, DNA replication, gene expression, and protein production properties. It gives high titres when used to produce many viral vectors such as oncoretroviruses and lentiviruses.

Zastosowanie

293T cell line human has been used:
  • for lentiviral particle production[3]
  • in the expression of eukaryotic proteins[4][5]
  • for small interfering RNA (siRNA) transfection[6]
  • for human immunodeficiency virus (HIV) transfection for pseudoviruses production[7]
  • as a host for the transfection of pLKO.1.-TRC-ACE2 plasmid to study the localization and role of NL63-N, a nucleocapsid protein of human coronavirus NL63, in cell cycle progression[8]

Działania biochem./fizjol.

Due to the presence of T antigen, 293T cell line favors simian virus 40 (SV40) plasmid replication as well as high protein expression.[2] It also possesses stem cell properties.[2] HEK293T is used for large-scale vector production, displays increased cell growth and is efficient for transfection experiments when compared to HEK293 cells.[9]

Opakowanie

UWAGA: Dla tego produktu może być dostępna zarówno linia komórkowa, jak i DNA z linii komórkowej. Wybierz -1VL lub VIAL dla komórek lub -DNA-5UG dla DNA.

Profil DNA

STR-PCR Data:

Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11
THO1: 7,9.3
TPOX: 11
vWA: 16,19

pożywka hodowlana

DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2-4 x 104 cells/cm2.

Inne uwagi

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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Certyfikaty analizy (CoA)

Lot/Batch Number

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Produkty

Eukaryotic cell lines aid in complex post-translational modifications and folding of recombinant proteins. Find suitable recombinant protein production cell lines.

Eukariotyczne linie komórkowe pomagają w złożonych modyfikacjach potranslacyjnych i fałdowaniu rekombinowanych białek. Znajdź odpowiednie linie komórkowe do produkcji białek rekombinowanych.

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

Questions

Reviews

Active Filters

  1. Denmark
    • Review 1
    • Votes 2
    2 out of 5 stars.

    Careful when odering! You might only order DNA, not viable cells!

    Extremely misleading product web page.
    As of today, a cell line is being described for sale, but they only sell extracted DNA. The whole web page is also listed under mammalian cell lines.
    Customer service clarified that viable cells have the product ID 12022001-1VL, for which there is no product entry (April 2024 to July 30th, 2024).
    So I waited for 2 months (!) to receive useless DNA.

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for your review. We are sorry to hear that your experience did not match your product expectations. We encourage customers who experience problems with our products to contact us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

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