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C6198

Sigma-Aldrich

Anti-Actin, α-Smooth Muscle - Cy3™ antibody, Mouse monoclonal

clone 1A4, purified from hybridoma cell culture

Synonim(y):

Monoclonal Anti-Actin, α-Smooth Muscle, Alpha-actin 2 Cy3, alpha-smooth muscle actin - Cy3™ antibody, SMA

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.43
białko sprzężone:
CY3 conjugate
application:
IHC (p)
klon:
1A4, monoclonal
reaktywność gatunkowa:
human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
citations:
896
metody:
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tonsil or appendix

pochodzenie biologiczne

mouse

Poziom jakości

200

białko sprzężone

CY3 conjugate

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

1A4, monoclonal

Formularz

buffered aqueous solution

masa cząsteczkowa

antigen ~42 kDa

reaktywność gatunkowa

human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake

warunki przechowywania

protect from light

metody

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tonsil or appendix

izotyp

IgG2a

numer dostępu UniProt

P62736

Zastosowanie

research pathology

Warunki transportu

wet ice

temp. przechowywania

2-8°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)

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Opis ogólny

Monoclonal Anti-Actin, α-Smooth Muscle (mouse IgG2a isotype) is derived from the 1A4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes of immunized BALB/c mice.
The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.

Immunogen

N-terminal synthetic decapeptide of α-smooth muscle actin.

Zastosowanie

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (2 papers)
Immunocytochemistry (3 papers)
Immunofluorescence (13 papers)
Immunohistochemistry (27 papers)
Western Blotting (2 papers)
Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.

Działania biochem./fizjol.

Smooth muscle actin is transiently coexpressed with sarcomeric α-actin during myogenesis in chicken and rat embryos. It has been found in the ventricular conducting tract of adult mammalian heart. It is expressed in leiomyomas, leiomyosarcomas and leiomyoblastomas, as well as in a proportion of rhabdomyosarcomas.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide, as a preservative.

Inne uwagi

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Informacje prawne

Cy is distributed under license from Amersham Biosciences Limited.
Cy3 is a trademark of Cytiva

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
0000357781
0000357780
0000312693
0000312692
0000310351

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Coexpression of alpha-sarcomeric actin, alpha-smooth muscle actin and desmin during myogenesis in rat and mouse embryos I. Skeletal muscle
Babai F, et al.
Differentiation, 44(2), 132-142 (1990)
Filipa Moraes et al.
Circulation research, 113(9), 1076-1086 (2013-07-31)
Arteriogenesis is the process of formation of arterial conduits. Its promotion is an attractive therapeutic strategy in occlusive atherosclerotic diseases. Despite the functional and clinical importance of arteriogenesis, the biology of the process is poorly understood. Synectin, a gene previously
Rebeca Ortega et al.
Arteriosclerosis, thrombosis, and vascular biology, 39(8), 1614-1628 (2019-07-12)
Abdominal aortic aneurysm (AAA) is a pathological condition of permanent vessel dilatation that predisposes to the potentially fatal consequence of aortic rupture. SGLT-2 (sodium-glucose cotransporter 2) inhibitors have emerged as powerful pharmacological tools for type 2 diabetes mellitus treatment. Beyond
Steven L Fabian et al.
The American journal of pathology, 180(4), 1441-1453 (2012-02-22)
The Hedgehog (Hh) signaling pathway regulates tissue patterning during development, including patterning and growth of limbs and face, but whether Hh signaling plays a role in adult kidney remains undefined. In this study, using a panel of hedgehog-reporter mice, we
Daniela Farkas et al.
PloS one, 9(2), e89810-e89810 (2014-03-04)
Successful curative treatment of severe pulmonary arterial hypertension with luminal obliteration will require a thorough understanding of the mechanism underlying the development and progression of pulmonary vascular lesions. But the cells that obliterate the pulmonary arterial lumen in severe pulmonary
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