C5838
Monoclonal Anti-Actin–Cy3 antibody produced in mouse
clone AC-40, purified immunoglobulin, buffered aqueous solution
Synonim(y):
Monoclonal Anti-Actin
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About This Item
Kod UNSPSC:
12352203
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
białko sprzężone
CY3 conjugate
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
AC-40, monoclonal
Postać
buffered aqueous solution
masa cząsteczkowa
antigen 42 kDa
reaktywność gatunkowa
human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat
metody
direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts
izotyp
IgG2a
Warunki transportu
wet ice
temp. przechowywania
2-8°C
docelowa modyfikacja potranslacyjna
unmodified
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Opis ogólny
Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin has six isoforms. Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. There are three α-actins (α-skeletal, α-cardiac and α-smooth muscle), one β-actin (β-nonmuscle) and two γ-actins (γ-smooth muscle and γ-nonmuscle).
The antibody recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms. It specifically labels actin in a wide variety of tissues and species. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel′s or Bouin′s solutions may also be used as fixatives.
Immunogen
synthetic actin C-terminal peptide, attached to a Multiple Antigen Peptide (MAP) backbone
Zastosowanie
Monoclonal Anti-Actin-Cy3 antibody has been used in immunofluorescent staining.
Monoclonal anti-actin antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading.
Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody.
Działania biochem./fizjol.
Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis.
Postać fizyczna
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Inne uwagi
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Informacje prawne
Distributed under license from Amersham Biosciences Limited.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
nwg
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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