C6079

Collagenase + protease inhibitor

Name: Collagenase + protease inhibitor
Brand: SIGMA
Price: 6530 PLN

2-5 FALGPA units/mg solid, ≥800 CDU/mg solid

Synonim(y):

Collagenase preparation, Protease inhibitor solution

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500 MG

6530,00 zł

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Informacje o tej pozycji

Numer WE:

3.4.24.3 (BRENDA, IUBMB)

NACRES:

NA.54

UNSPSC Code:

12352204

Form:

solid

Solubility:

water: soluble

Storage temp.:

−20°C

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Pozwól nam pomóc

biological source

bacterial (Clostridium histolyticum)

form

solid

specific activity

≥800 CDU/mg solid, 2-5 FALGPA units/mg solid

mol wt

68-130 kDa

solubility

water: soluble

shipped in

dry ice

storage temp.

−20°C

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Pokaż różnice

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Ta pozycja	C2799	C7926	C2674
Sigma-Aldrich C6079 Collagenase + protease inhibitor	Sigma-Aldrich C2799 Collagenase from Clostridium histolyticum	Sigma-Aldrich C7926 Collagenase from Clostridium histolyticum	Sigma-Aldrich C2674 Collagenase from Clostridium histolyticum
form solid	form powder	form powder	form lyophilized powder
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
biological source bacterial (Clostridium histolyticum)	biological source Clostridium histolyticum	biological source Clostridium histolyticum	biological source Clostridium histolyticum
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
shipped in dry ice	shipped in dry ice	shipped in -	shipped in ambient
specific activity ≥800 CDU/mg solid	specific activity ≥4 FALGPA units/mg solid, ≥700 CDU/mg solid (CDU = collagen digestion units)	specific activity ≥2.0 FALGPA units/mg solid	specific activity ≥125 CDU/mg solid (CDU = collagen digestion units), 0.5-5.0 FALGPA units/mg solid

Application

Collagenase with protease inhibitor has been used in a study to assess the association of immune system gene polymorphisms with quantitative features. It has also been used in a study to investigate the quantitative structure-activity relationship study on Clostridium histolyticum collagenase inhibitors. The enzyme from sigma has been used in the isolation of porcine pancreatic islets.[1] It has also been used in the isolation of pancreatic-infiltrating lymphocytes from mice.[2]

Biochem/physiol Actions

Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four grams of calcium (Ca²⁺) per mole of the enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL are used for digestion. The duration of reaction can vary from 15 minutes to several hours for satisfactory cell dissociation without causing too much cell death. Zn²⁺ is required for activity. This product is used if the collagenase does not require a significant protease activity.

Analysis Note

Also contains clostripain and non-specific neutral protease activities.

Other Notes

Selected lots of collagenase Type XI blended with protease inhibitor to limit the tryptic enzyme activities in pancreatic tissue. The formulation was developed through collaboration with several outside laboratories.

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related product

Numer produktu

Opis

Cennik

D4434

Dichloromethylenediphosphonic acid disodium salt

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H302 + H312 + H332,H317,H334

pcodes

P261 - P264 - P271 - P280 - P301 + P312 - P302 + P352 + P312

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Resp. Sens. 1 - Skin Sens. 1

Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certyfikaty analizy (CoA)

Lot/Batch Number

0000334536

0000270820

0000246551

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

QSAR analysis of some 5-amino-2-mercapto-1,3,4-thiadiazole based inhibitors of matrix metalloproteinases and bacterial collagenase.

Ashutosh Jamloki et al.

Bioorganic & medicinal chemistry letters, 16(14), 3847-3854 (2006-05-10)

A quantitative structure-activity relationship (QSAR) study has been performed on 5-amino-2-mercapto-1,3,4-thiadiazole based inhibitors of matrix metalloproteinases (MMPs) and a bacterial collagenase known as Clostridium histolyticum collagenase (ChC) to understand the structural features influencing the affinity of these inhibitors towards the

Association of immune system gene polymorphisms with quantitative features which are pathogenetically important in chronic viral hepatitis.

Goncharova, I., et al.

Molecular Biology, 42, 242-246 (2008)

Double negative (CD3+ 4- 8-) TCR alphabeta splenic cells from young NOD mice provide long-lasting protection against type 1 diabetes.

Beverly Duncan et al.

PloS one, 5(7), e11427-e11427 (2010-07-14)

Double negative CD3(+)4(-)8(-) TCR alphabeta splenic cells (DNCD3) can suppress the immune responses to allo and xenografts, infectious agents, tumors, and some autoimmune disorders. However, little is known about their role in autoimmune diabetes, a disease characterized by the reduction

A quantitative structure-activity relationship study on Clostridium histolyticum collagenase inhibitors: roles of electrotopological state indices.

S P Gupta et al.

Bioorganic & medicinal chemistry, 11(14), 3065-3071 (2003-06-24)

A quantitative structure-activity relationship (QSAR) study has been made on eight different series of Clostridium histolyticum collegenase (ChC) inhibitors. These series are comprised of four different groups of sulfonylated amino acids and their corresponding hydroxamates. In each series, the inhibition

CCL5 mediates CD40-driven CD4+ T cell tumor infiltration and immunity.

Austin P Huffman et al.

JCI insight, 5(10) (2020-04-24)

The role CD4+ T cells play in tumor immunity is less well appreciated than the cytotoxic role of CD8+ T cells. Despite clear evidence for CD4+ T cell dependency across multiple immunotherapies, the mechanisms by which CD4+ T cells infiltrate

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Protokoły

Enzymatic Assay of Collagenase (EC 3.4.24.3) using FALGPA (N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala)

To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

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