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Key Documents

C-12576

Sigma-Aldrich

Human Uterine Smooth Muscle Cells (HUtSMC)

500,000 proliferating cells

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About This Item

Kod UNSPSC:
41106514
NACRES:
NA.81

pochodzenie biologiczne

human uterus (myometrium)

opakowanie

pkg of 500,000 cells

morfologia

(smooth muscle)

metody

cell culture | mammalian: suitable

Warunki transportu

ambient

temp. przechowywania

room temp

Opis ogólny

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Pochodzenie linii komórkowej

Uterus

Zastosowanie

Primary Human Uterine Smooth Muscle Cells (HUtSMC) are isolated from the myometrium (the middle layer of the uterine wall) and are provided in a proliferating format. HUtSMC stain positive for smooth muscle α-actin. During pregnancy, hormones and growth factors cause an extensive hypertrophy of uterine smooth muscle cells. This and other morphological and biochemical effects on the uterus can be studied in vitro using HUtSMC.

Jakość

Rigid quality control tests are performed for each lot of Human Smooth Muscle Cells. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. smooth muscle a-actin, are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Ostrzeżenie

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Rutyna subkultury

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Inne uwagi

Recommended Plating Density: 7500 - 10000 cells per cm2Passage After Thawing: P2Tested Markers: Smooth muscle alpha-actin positiveGuaranteed population doublings: >15

Polecane produkty

Recommended Primary Cell Culture Media:Link

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Produkty

Smooth muscle human primary cells isolated from blood vessels and hollow organs, plus smooth muscle cell growth media. Protocols on media handling, cell passaging and product use.

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