The major advantage of the colloidal form is that it gives less background staining that the "regular" non-colloidal Coomassie Brilliant Blue G and Brilliant Blue R stains. Sensitivity can be markedly increased by double staining, first with Coomassie Brilliant Blue G Colloidal, then Rapid Silver Stain.
B2025
Brilliant Blue G - Colloidal Concentrate
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Informacje o tej pozycji
NACRES:
NA.85
UNSPSC Code:
12352200
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Ta pozycja | |||
|---|---|---|---|
| form solution | form liquid | form powder | form - |
| storage temp. room temp | storage temp. 2-8°C | storage temp. room temp | storage temp. no temp limit |
| Quality Level 200 | Quality Level - | Quality Level 200 | Quality Level 100 |
General description
Coomassie Brilliant Blue stain is widely used for the detection of proteins in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE).[1] Staining with brilliant blue G is called as blue silver staining due to its improved sensitivity close to silver staining method. Coomassie Brilliant Blue G stain associates with proteins via hydrophobic interaction.[2] It is effective in staining anterior capsule in cornea with relatively less toxic effects.[3]
Application
Preparation Note
The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 liter with 800 mL water.
Analysis Note
This product has been tested for suitability on SDS-PAGE.
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Opis
Cennik
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hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Klasa składowania
12 - Non Combustible Liquids
Wykazy regulacyjne
Wykazy regulacyjne dotyczą głównie produktów chemicznych. Można w nich podawać ograniczoną liczbę informacji na temat produktów niechemicznych. Brak wpisu oznacza, że żaden ze składników nie znajduje się w wykazie. Użytkownik odpowiada za zagwarantowanie bezpiecznego i zgodnego z prawem stosowania produktu.
7783-20-2
CAS No.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Proteomic analysis of ovarian cancer tumor fluid is a rich source of potential biomarkers
Poersch A, et al.
Journal of Proteomics and Bioinformatics, 5(2), 2-2 (2014)
Elena Barbieri et al.
Journal of aging research, 2011, 845379-845379 (2011-06-02)
This study describes mitochondrial behaviour during the C2C12 myoblast differentiation program and proposes a proteomic approach to mitochondria integrated with classical morphofunctional and biochemical analyses. Mitochondrial ultrastructure variations were determined by transmission electron microscopy; mitochondrial mass and membrane potential were
Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis
Candiano G, et al.
Electrophoresis, 25(9), 1327-1333 (2004)
A proteomic signature of ovarian cancer tumor fluid identified by highthroughput and verified by targeted proteomics
Poersch A, et al.
Journal of proteomics, 145(2), 226-236 (2016)
Roy Auty et al.
The Journal of biological chemistry, 279(48), 49973-49981 (2004-09-28)
The basal transcription factor TFIID is composed of the TATA-binding protein (TBP) and 14 TBP-associated factors (TAFs). Although TBP alone binds to the TATA box of DNA and supports basal transcription, the TAFs have essential functions that remain poorly defined.
Protokoły
Brilliant Blue G-Colloidal Concentrate stains proteins in IEF, PAGE, and SDS-PAGE gels after electrophoresis, enhancing sensitivity with protein fixation.
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How is Product B2025, Brilliant Blue G, better than other Coomassie Blue dyes?
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Can you use Product B2025, Brilliant Blue G, with nitrocellulose?
1 answer-
Use with nitrocellulose is not recommended, due to the methanol.
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What is the solution stability of Product B2025, Brilliant Blue G?
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Once diluted the solution should be stable indefinitely. It should be stored in the refrigerator and shaken well before each use. The solution tends to precipitate on standing and heat accelerates precipitation. Therefore, do not leave the bottle at room temperature overnight.
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What is the detection limit of Product B2025, Brilliant Blue G?
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The detection limit for proteins is about 50 ng of protein. However, proteins pick up stains differently so this is a general description and may not apply to all proteins
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How would you recommend that I reconstitute Product B2025, Brilliant Blue G?
1 answer-
One bottle of the product makes 1 liter of working solution. The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 Liter with 800 mL water.
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