With product A5955, we recommend storing the product at all times unmixed, at -20 °C. We only have one limited storage condition tested when in solution at 37 °C, and it appears to be stable for up to 3 days. It is best to produce the product each time and use it immediately.
A5955
Antibiotic Antimycotic Solution (100×), Stabilized
suspension, suitable for cell culture, BioReagent
Synonim(y):
Penicillin-Streptomycin-Amphotericin B Suspension
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Wybierz wielkość
20 ML
200,00 zł
100 ML
306,00 zł
200,00 zł
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Pozwól nam pomócNazwa produktu
Antibiotic Antimycotic Solution (100×), Stabilized, with 10,000 units penicillin, 10 mg streptomycin and 25 μg amphotericin B per mL, 0.1 μm filtered, BioReagent, suitable for cell culture
Quality Level
sterility
0.1 μm filtered
product line
BioReagent
form
suspension
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
yellow
antibiotic activity spectrum
Gram-negative bacteria, Gram-positive bacteria, fungi, yeast
application(s)
agriculture
mode of action
cell wall synthesis | interferes
shipped in
dry ice
storage temp.
−20°C
Powiązane kategorie
1 of 4
Ta pozycja | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| antibiotic activity spectrum Gram-negative bacteria, fungi, Gram-positive bacteria, yeast | antibiotic activity spectrum Gram-negative bacteria, Gram-positive bacteria | antibiotic activity spectrum Gram-negative bacteria, Gram-positive bacteria | antibiotic activity spectrum fungi, yeast |
| mode of action cell wall synthesis | interferes | mode of action cell wall synthesis | interferes, protein synthesis | interferes | mode of action cell wall synthesis | interferes, protein synthesis | interferes | mode of action cell membrane | interferes |
| product line BioReagent | product line BioReagent | product line BioReagent | product line BioReagent |
| Quality Level 200 | Quality Level 300 | Quality Level 300 | Quality Level 200 |
| form suspension | form liquid | form liquid | form liquid |
General description
Antibiotic Antimycotic Solution is formulated to contain 10,000 units penicillin, 10 mg streptomycin and 25μg Amphotericin B per mL, and is solubilized in a proprietary citrate buffer. It is recommended for use in cell culture applications at 10mL/L. Antibioticantimycotic solution is generally used to prevent the growth of harmful microorganisms including bacteria and fungi in cell culture media.
Application
Antibiotic antimycotic solution has been used:
- as a supplement in different types of cell culture media.[1][2][3]
- as a component of phosphate buffer saline (PBS) sterile solution to prevent contamination of periodontal ligament fibroblasts (PDLF) tissues that are isolated and cultured in the experiments.[4]
- as a component of storage solution for preserving tracheal segments harvested for subsequent experimental investigation.
Biochem/physiol Actions
Mode of Action: Penicillin acts by inhibiting bacterial cell-wall synthesis. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome and causes miscoding. Amphotericin B interferes with fungal membrane permeability by forming channels in the membranes and causing small molecules to leak out.
Antimicrobial spectrum: Includes Gram-negative bacteria, Gram-positive bacteria, fungi and yeasts.
Antimicrobial spectrum: Includes Gram-negative bacteria, Gram-positive bacteria, fungi and yeasts.
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Numer produktu
Opis
Cennik
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Klasa składowania
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Produkty
Prevent fungal, yeast, and mold contamination in cell cultures. Discover the best antifungal agent for your cultures with the extensive Sigma® antifungal collection.
Powiązane treści
Learn how to use our cell culture tested, ready-to-use MilliShot™ single dose antibiotic solutions, conveniently packaged in one-time use vials.
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| A5955-20ML | 04061838447012 |
| A5955-100ML | 04061838447005 |
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I am currently using your Antibiotic Antimycotic Solution (Product No. A5955) and would like to know the recommended storage conditions and stability of the product once it has been diluted to 1× working concentration .
1 answer-
Helpful?
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Hello, Could you please confirm that the antibiotic/antimycotic is tested for sterility as this does not appear on your CoA.
1 answer-
This product is sterile filtered through a 0.1 μm membrane. However, sterility testing is not performed on the finished product.
Helpful?
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I am looking at the SDS for product number A5955, Antibiotic Antimycotic soln. I do not see Amphotericin B in composition of ingredients (3.2 mixtures). I want to know why. Thank you.
1 answer-
Section 3 of SDS shows only the hazardous component information. Product# A5955 contains microgram amounts of Amphotericin B. At this concentration it is non-hazardous, hence section 3 of SDS does not list Amphotericin B.
Helpful?
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