A2172

Anti-α-Actin-1 (ACTA1) antibody

mouse monoclonal, 5C5

• Synonim(y): Mysz anty-aktyna
• Numer MDL: MFCD00164521
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• Nazwa produktu: Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse, clone 5C5, ascites fluid
• pochodzenie biologiczne: mouse
• białko sprzężone: unconjugated
• forma przeciwciała: ascites fluid
• rodzaj przeciwciała: primary antibodies
• klon: 5C5, monoclonal
• zawiera: 15 mM sodium azide
• reaktywność gatunkowa: rabbit, carp, rat, sheep, guinea pig, human, snake, bovine, frog
• metody: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human skeletal and cardiac muscle; immunohistochemistry (frozen sections): suitable using human skeletal and cardiac muscle; indirect ELISA: suitable; western blot: suitable
• izotyp: IgM
• numer dostępu UniProt: P68032; P68133
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... ACTA1(58) , ACTC1(70) ; mouse ... Rwdd2a(69519) ; rat ... Acta1(29437) , Actc1(29275)

Opis

Opis ogólny

Monoclonal Anti-α-Sarcomeric Actin (mouse IgM isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin is one of the most conserved eukaryotic proteins and the isoforms show >90% overall sequence homology. It is expressed in mammals and birds as six isoforms. Four of the six represent differentiation markers of muscle tissues. The other two are found in practically all cells.

Immunogen

rabbit striated muscle actin.

Zastosowanie

Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse has also been used

• immunofluorescence[1]
• double immunostaining
• double labelling terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) immunofluorescence staining.

Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.

Mouse monoclonal anti-actin (alpha-sarcomeric) antibody has been used for western blot, and immunohistochemistry applications.

Działania biochem./fizjol.

Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin.

Postać fizyczna

The product is provided as ascites fluid containing 0.1% sodium azide as a preservative.

Inne uwagi

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable