SRP3276

SDF-1 ALPHA human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonim(y): CXCL12, PBSF
• Kod UNSPSC: 12352202
• NACRES: NA.32

Właściwości

• pochodzenie biologiczne: human
• rekombinowane: expressed in E. coli
• Próba: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Formularz: lyophilized
• siła działania: 20.0-80.0 ng/mL
• masa cząsteczkowa: 8.0 kDa
• opakowanie: pkg of 10 μg
• zanieczyszczenia: <0.1 EU/μg endotoxin, tested
• kolor: white
• numer dostępu UniProt: P48061
• Warunki transportu: wet ice
• temp. przechowywania: −20°C
• informacje o genach: human ... CXCL12(6387)

Opis

Opis ogólny

SDF-1α (stromal cell-derived factor 1 α) and β are stromal derived CXC chemokines, and signal through the CXCR4 (C-X-C motif chemokine receptor).[1][2][3] SDF1α is an isoform of CXCL12 (C-X-C motif chemokine 12, also referred to as SDF-1).[2] The gene is mapped to human chromosome 10q11.[4] Recombinant human SDF-1a is an 8.0kDa protein containing 68 amino acid residues.

Zastosowanie

SDF-1α (stromal cell-derived factor 1 α) human has been used to study the role of SDF-1α in cell migration and survival after TGF (transforming growth factor)-β-induced EMT (epithelial–mesenchymal transition) in hepatoma cells.[5] It has been used to study podia formation, migration, proliferation and cell-cell adhesion of human hematopoietic progenitor cells in presence of SDF-1α.[6]

SDF-1α (stromal cell-derived factor 1 α) human has been used in the chemotaxis assay of neural crest cells (NCCs)[7] and as a component of Dulbecco′s modified Eagle′s medium (DMEM) in migration assay of human mesenchymal stem cells (hMSCs).[8]

Działania biochem./fizjol.

SDF-1α (stromal cell-derived factor 1 α) and β chemoattract B and T cells, and have been shown to induce migration of CD34+ stem cells.[9][10] SDF-1 is mainly expressed in the inflamed and failing myocardium.[11] It is involved in inflammation, proliferation, tumorigenicity and metastasis.[12] The SDF-1 proteins exert HIV (human immunodeficiency virus) suppressive activity in cells expressing the CXCR4 (C-X-C motif chemokine receptor 4).[13] It is upregulated in breast carcinoma, ovarian carcinoma and papillary thyroid carcinoma.[1] It enhances angiogenesis in the ischemic tissue. SDF1 is also involved in neuroprotection after ischemic stroke with the help of CXCR4.[2]

Postać fizyczna

Lyophilized with no additives.

Rekonstytucja

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable