This item is a multi-component product. A CAS number cannot be applied to this type of product. The Safety Data Sheet document includes the individual CAS numbers of those components that may be potentially hazardous.
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D8537
Dulbecco′s Phosphate Buffered Saline
Dulbecco’s Phosphate Buffered Saline
1 of 6 reviewers received a sample product or took part in a promotion
liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
Synonim(y):
DPBS
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About This Item
Informacje o cenach i dostępności nie są obecnie dostępne.
Polecane produkty
Nazwa produktu
Dulbecco′s Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
Poziom jakości
sterylność
sterile-filtered
Formularz
liquid
metody
cell culture | mammalian: suitable
zanieczyszczenia
endotoxin, tested
komponenty
glucose: no (Dextro)
sodium pyruvate: no
Warunki transportu
ambient
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Opis ogólny
Dulbecco’s Phosphate Buffered Saline (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS buffer is used to irrigate, wash, and dilute mammalian cells while maintaining osmotic balance. It provides water and inorganic ions essential for normal cell metabolism. DPBS with calcium and magnesium facilitates cell binding and clumping. Dulbecco′s PBS without these ions can be used to wash and rinse suspended cells. Phosphate buffering maintains the pH in the physiological range.
Zastosowanie
Dulbecco′s Phosphate Buffered Saline has been used:
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Numer produktu
Opis
Cennik
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Protokoły
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.
Protokół hodowli komórkowej do zamrażania komórek o wysokiej żywotności przy użyciu odczynników do kriokonserwacji, takich jak DMSO. Bezpłatny podręcznik do pobrania.
Technical information for working with rat skeletal muscle cells including thawing, subculturing and cryopreservation
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Bonjour, pourriez-vous m'indiquer le numéro de CAS de la solution de phosphate saline dulbecco sous forme liquide
1 answer-
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Hi, is this product GMP-grade or are GMP-garde material is used for the production of this?
1 answer-
This product is not GMP-grade. Unfortunately. a GMP-grade version of this product is not available.
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What is the concentration of the product in Molarity?
1 answer-
This is a multi-component formulation. A 'molarity' is not reported for this type of product. See below for the list of components:
Product D8537 contains:
Potassium chloride: 0.2 g/l
Potassium phosphate monobasic: 0.2 g/l
Sodium chloride: 8 g/l
Sodium Phosphate dibasic: 1.15 g/lPlease see the link below for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/124/464/d8537for.pdfHelpful?
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What is the bottle thread type on the 1L bottle (D8537-1L)
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The product is packaged in a square PET bottle with an HDPE cap. The cap specifications are as follows:
Thread Type 38-430
Thread Diameter 37.3 mmHelpful?
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What is the formulation for Product D8537, Dulbecco's Phosphate?
1 answer-
The formulation for 1 liter is 8 g Sodium Chloride, 0.2 g Potassium Phosphate, monobasic, 1.15 g Sodium Phosphate, dibasic, and 0.2 g Potassium Chloride.
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What is a Salt Toxicity Test?
1 answer-
The Cell Culture assay is called a Salt Toxicity Test (previously called Cell Culture Salt Testing). A more complete description is below:Cells are harvested and counted. They are resuspended in DPBS and incubated at 37°C, 5% CO2 for 1 hour. The cells are counted and %viability determined. The cells are then cultured for an additional 7 hours (total of 8 hours). The cells are counted again and the % viability determined. The viability at 1 hr has to be >95% of the original viability. The viability at 8 hrs must be >75% of the original viability.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the pH of a solution of Product D8537, Dulbecco's Phosphate?
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The pH will be in the range of 7.1-7.5, according to the specification sheet.
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Is Product D8537, Dulbecco's Phosphate, a 1X or 10 X concentration?
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This is a 1X concentration. The 10X concentration is listed under product D1408.
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