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Merck

64958

2-Methoxy-2,4-diphenyl-3(2H)-furanone

suitable for fluorescence, ≥98.0% (HPLC)

Synonim(y):

2,4-Diphenyl-2-methoxy-3(2H)-furanone, MDPF

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Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):
C17H14O3
Numer CAS:
50632-57-0
Masa cząsteczkowa:
266.29
UNSPSC Code:
12352108
NACRES:
NA.32
PubChem Substance ID:
329760016
MDL number:
MFCD00036834
Beilstein/REAXYS Number:
1288529

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InChI

1S/C17H14O3/c1-19-17(14-10-6-3-7-11-14)16(18)15(12-20-17)13-8-4-2-5-9-13/h2-12H,1H3

SMILES string

COC1(OC=C(C1=O)c2ccccc2)c3ccccc3

InChI key

BLWINLJDTOJSRU-UHFFFAOYSA-N

assay

≥98.0% (HPLC)

form

solid

fluorescence

λex 384 nm; λem 472 nm in acetonitrile (after derivatization with hexylamine)

suitability

suitable for fluorescence

storage temp.

2-8°C

Quality Level

100

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Ta pozycja
B-0998.41502277487
2-Methoxy-2,4-diphenyl-3(2H)-furanone suitable for fluorescence, ≥98.0% (HPLC)

Sigma-Aldrich

64958

2-Methoxy-2,4-diphenyl-3(2H)-furanone

2-Butanone-2,4-DNPH certified reference material, vial of 100 mg, Cerilliant®

Supelco

B-099

2-Butanone-2,4-DNPH

2,3-Dimethylmaleic anhydride for synthesis

Sigma-Aldrich

8.41502

2,3-Dimethylmaleic anhydride

2-Methyltetrahydro-3-furanone 98%

Sigma-Aldrich

277487

2-Methyltetrahydro-3-furanone

suitability

suitable for fluorescence

suitability

-

suitability

-

suitability

-

fluorescence

λex 384 nm; λem 472 nm in acetonitrile (after derivatization with hexylamine)

fluorescence

-

fluorescence

-

fluorescence

-

form

solid

form

-

form

crystals

form

liquid

Quality Level

100

Quality Level

300

Quality Level

200

Quality Level

100

assay

≥98.0% (HPLC)

assay

-

assay

≥98.0% (GC)

assay

98%

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-30°C

storage temp.

-

Application

2-Methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) is used as a fluorescence reagent to derivatize primary and secondary amines on molecules such as proteins. MDPF derivatized molecules can be detected during analytical and separation procedures including chromatography, electrophoresis and zymography.

Other Notes

Reagent for the derivatization of primary and secondary amines for HPLC; highly fluorescent derivatives are formed[1][2]; Pre-column derivatization of amines[3]; Fluorescent labeling of proteins before SDS-PAGE[4]
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pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
BCCL1534
BCBT5586
BCBS6087V
BCBP1051V
BCBK6273V

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

R C Schaeffer et al.
International journal of microcirculation, clinical and experimental, 11(2), 181-201 (1992-05-11)
We studied the macromolecular size-selective transport characteristics of polycarbonate (PC) filters with defined pore radius (rp; 15,000 to 400 A) as well as 2,000 A rp PC filter-bovine pulmonary artery endothelial cell (EC) monolayer sandwich under zero hydrostatic pressure conditions
S K Williams et al.
Journal of cellular physiology, 120(2), 157-162 (1984-08-01)
The transport of proteins across continuous capillary endothelium is believed to be mediated by micropinocytic vesicles which shuttle molecules between the lumenal and abluminal plasma membrane. We have studied the ability of capillary endothelial cells isolated from rat epididymal fat
F J Alba et al.
Electrophoresis, 19(14), 2407-2411 (1998-11-20)
The fluorogenic dye 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) has been used for the detection of total protein patterns on polyvinylidene difluoride (PVDF) membranes. Fluorescent staining of protein bands on membranes with this covalent dye is completed in 20 min. Wet membranes are translucent
B W Falk et al.
Analytical biochemistry, 144(2), 537-541 (1985-02-01)
Fluorescent labeling of proteins was found to be a very sensitive and reliable alternative to conventional methods for monitoring proteins on Western blots. Proteins were labeled with 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) before SDS-PAGE. After electrophoresis and subsequent electro-blotting the fluorescent-labeled proteins were
Danqing Min et al.
Electrophoresis, 27(2), 357-364 (2005-12-07)
Measurement of matrix metalloproteinases (MMPs) and their specific tissue inhibitors of metalloproteinases (TIMPs) by the techniques of zymography and reverse zymography provide useful information regarding the status of matrix accumulation or breakdown. This report describes the use of 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF)
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