POLYDIC-RO

Poly[d(I-C)]

lyophilized, pkg of 10 U (10108812001 [A₂₆₀ units]), pkg of 50 U (11219847001 [A₂₆₀ units])

• Synonim(y): Poly-deoxy-inosinic-deoxy-cytidylic acid
• Kod UNSPSC: 12352200

Właściwości

• opis: Poly-deoxy-inosinic-deoxy-cytidylic acid
• Poziom jakości: 100
• Formularz: lyophilized
• masa cząsteczkowa: 28814 bp
• opakowanie: pkg of 10 U (10108812001 [A₂₆₀ units]); pkg of 50 U (11219847001 [A₂₆₀ units])
• producent / nazwa handlowa: Roche
• kolor: white
• mp: 54 °C (NaCl, 0.1 M, pH 7.0)
• rozpuszczalność: water: soluble
• stosunek absorbancji: A_{290/260 nm} 0.24-0.32; A_{280/260 nm} 0.61-0.67; A_{250/260 nm} 1.18-1.30
• temp. przechowywania: −20°C

Opis

Zastosowanie

Poly[d(I-C)] is used as a template for RNA polymerases, and as a DNA model for biophysical investigations.
It is also used as a primer template with Kornberg DNA polymerase for the determination of small amounts of dITP, dGTP, and dCTP. Poly(dI-dC) has been used in electrophoretic mobility shift assays (EMSA).[1]

Cechy i korzyści

Contents
Lyophilizate, sodium salt, base ratio 1:1, alternating base sequence

Jakość

Typical analysis:
Absorbance determination A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀

Sekwencja

Chain Length approx. 20,000 bp

In an aqueous solution the DNA itself is a salt (the acid groups of the phosphate back bone have a negative charge and are saturated by positive sodium ions) i.e. there is no additional salt in solution. The structural formula which shows the negative 0-groups in the phosphate backbone is available. The same number of positive sodium ions must be dissociated in solution as counter ions.

As the sodium ions are part of the product and saturate the phosphate groups, the lyophilisate is salt-free.

Definicja jednostki

1 A₂₆₀ unit corresponds to 50 μg ds DNA.

Unit Conversion: 1 A₂₆₀ unit corresponds to 50 μg ds DNA.

Uwaga dotycząca przygotowania

Activator: Polymines (spermine, spermidine)
Storage conditions (working solution): Solution is stable to at least 12 months if stored at 2 to 8 °C, contact with DNase or bacteria is to avoid.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable