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11669915001
Roche
Test ELISA na proliferację komórek, BrdU (chemiluminescencyjny)
Synonim(y):
BrdU, proliferacja komórek
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Wybierz wielkość
1000 TESTS
3810,00 zł
3810,00 zł
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Wybierz wielkość
Zmień widok
1000 TESTS
3810,00 zł
About This Item
Kod UNSPSC:
41116158
3810,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Polecane produkty
zastosowanie
sufficient for ≤1,000 tests
Poziom jakości
producent / nazwa handlowa
Roche
metody
ELISA: suitable
metoda wykrywania
chemiluminescent
Warunki transportu
wet ice
temp. przechowywania
2-8°C
Powiązane kategorie
Opis ogólny
Chemiluminescent immunoassay for the quantification of cell proliferation, based on the measurement of BrdU incorporation during DNA synthesis: a nonradioactive alternative to the [3H]-thymidine incorporation assay.
Specyficzność
The antibody conjugate reacts with the thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) and with BrdU incorporated into DNA. For binding to BrdU incorporated into the DNA, the BrdU-labeled DNA has to be denatured. The antibody does not cross-react with any endogenous cellular components such as thymidine, uridine, or DNA.
Zastosowanie
Cell Proliferation ELISA, BrdU (chemiluminescent) has been used:
- for bromodeoxyuridine (BrdU) proliferation assay in ReN and primary astrocytes[1]
- to test the effect on imatinib on the proliferation of neuroblastoma cells using BrdU incorporation assay[2]
- to check the effect of palbociclib on cell cycle inhibition using BrdU incorporation in pancreatic patient-derived primary cell lines[3]
The Cell Proliferation ELISA, BrdU (chemiluminescent) belongs to the second, improved generation of kits for measuring DNA synthesis. It is a precise, fast, and simple nonradioactive alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell systems. For example:
- Detection and quantification of cell proliferation induced by growth factors and cytokines[4][5]
- Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation in environmental and biomedical research, and in the food, cosmetic, and pharmaceutical industries
- Measurement of the immunoreactivity of lymphocytes, stimulated by mitogens or antigens
- Analysis of the chemosensitivity of tumor cells to different cytostatic drugs in medical research
Opakowanie
1 kit containing 7 components.
Komponenty
- BrdU Labeling Reagent, 1,000x conc
- FixDenat
- Anti-BrdU-POD 4. Antibody Dilution Solution
- Washing Buffer PBS, 10x conc.
- Substrate Component A
- Substrate Component B
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
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Tylko elementy zestawu
Numer produktu
Opis
- BrdU Labeling Reagent
- FixDenat ready-to-use
- Anti-BrdU-peroxidase antibody
- Antibody Dilution Solution ready-to-use
- ready-to-use 10x concentrated
- Substrate Component A
- Substrate Component B
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Irrit. 2 - Flam. Liq. 2 - Muta. 1B - Skin Sens. 1
Kod klasy składowania
3 - Flammable liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
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Produkty
Cell cycle regulates vital processes like DNA repair, cancer prevention. Four stages: G1, S, G2, M. NTPs don't permeate membranes.
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
Testy komórkowe do proliferacji komórek (BrdU, MTT, WST1), żywotności komórek i eksperymentów cytotoksyczności do zastosowań w badaniach nad rakiem, neuronauką i komórkami macierzystymi.
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
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