11412272001

Transformujący czynnik wzrostu-β1, ludzki (hTGF-β1)

recombinant (CHO cells)

• Synonim(y): hTGF
• Kod UNSPSC: 12352207

Właściwości

• pochodzenie biologiczne: human
• Poziom jakości: 100
• rekombinowane: expressed in CHO cells
• Próba: >95% (SDS-PAGE)
• Formularz: solution
• siła działania: <0.1 ng/mL EC₅₀
• masa cząsteczkowa: 25,000 Da
• opakowanie: pkg of 1 mL (1 μg)
• producent / nazwa handlowa: Roche
• warunki przechowywania: avoid repeated freeze/thaw cycles
• zanieczyszczenia: <0.1 EU/μgtested (LAL test)
• numer dostępu UniProt: P01137
• temp. przechowywania: −20°C
• informacje o genach: human ... TGFB1(7040)

Opis

Opis ogólny

Recombinant, human TGF- β1 is produced in CHO (Chinese Hamster Ovary) cells and purified by standard chromatographic techniques.
Solution in PBS and 1 mg/ml BSA, filtered through 0.2 μm pore size membrane.

Specyficzność

Species specificity: Transforming growth factor-β1 (TGF-β1) is effective on human and mouse cells.
Specific activity/EC₅₀: <0.1 ng/ml, at least the same specific activity (EC<SUB>50) compared to the indicated standard is guaranteed.

Zastosowanie

Transforming growth factor-β1, human (hTGF-β1) has been used to explore function and underlying mechanism of periostin (PN) during orthodontic periodontal ligament remodeling.[1]

Działania biochem./fizjol.

Transforming Growth Factor-β1, human (hTGF-β1), is a multifunctional growth factor affecting many functions in a variety of different cells.[2]

Transforming growth factor-β (TGF-β) family comprises of a group of multifunctional cytokines which are involved in controlling apoptosis, growth and differentiation of cells, and play important role during embryo development.[3]

Jakość

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
(Note: 1 EU corresponds to 0.1 ng)

Sekwencja

Chain Length 2 x 112 AA

Two identical polypeptide chains (112 amino acids) connected by disulfide bridges, identical to natural hTGF-β1.

Definicja jednostki

EC₅₀ definition: The concentration of hTGF-β1 that is required to mediate half maximal inhibition of DNA synthesis (BrdU incorporation) with Mv1-Lu cells.

Uwaga dotycząca przygotowania

Working concentration: 0.01 - 10 ng/ml
Recombinant, human TGF-β 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml.
Working solution: Dilute the concentrated TGF-β1 solution (1 μg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%.
Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): nwg
• Temperatura zapłonu (°F): does not flash
• Temperatura zapłonu (°C): does not flash