This product is intended for single use. Repeated usage may result in declining performance.
UFC9030
Amicon® Ultra Centrifugal Filter, 30 kDa MWCO
Amicon® Ultra Centrifugal Filter, 30 kDa MWCO
sample volume 15 mL, regenerated cellulose membrane, MWCO 30 kDa
Synonim(y):
15 mL sample volume, 30 kDa MWCO, Centrifugal concentrator
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8 PKG
507,00 zł
24 PKG
1580,00 zł
96 PKG
5710,00 zł
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8 PKG
507,00 zł
24 PKG
1580,00 zł
96 PKG
5710,00 zł
About This Item
Kod UNSPSC:
41104916
eCl@ss:
36100101
NACRES:
NB.24
Polecane produkty
Materiały
polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
Poziom jakości
sterylność
non-sterile
linia produktu
Amicon®
opakowanie
pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg
producent / nazwa handlowa
Amicon® Ultra
sustainability
Greener Alternative Product
Parametry
15 mL sample volume
metody
protein extraction: suitable
protein purification: suitable
Długość
12.1 cm (4.807 in.)
Średnica
2.97 cm
powierzchnia filtracyjna
7.6 cm2
pojemność
15 mL
pojemność robocza
≤15 mL
15 mL
wielkość porów
30 kDa MWCO
kategoria ekologicznej alternatywy
Warunki transportu
ambient
Opis ogólny
Amicon® Ultra 15 mL centrifugal devices with Ultracel® 30,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Ovalbumin (45 kDa) at 1 mg/ml initial concentration is retained at >95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Zastosowanie
- Ultrafiltration with 30,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
Cechy i korzyści
- Ultracel® regenerated cellulose membrane with 30 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipettor sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
Postać fizyczna
Color Code: Clear with pink cap
Informacje prawne
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
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Produkty
Filtration methods and membrane filter selection in nanoparticle purification and production.
Metody filtracji i wybór filtrów membranowych w oczyszczaniu i produkcji nanocząstek.
Poznaj zasady działania membran ultrafiltracyjnych i korzyści związane z przygotowywaniem próbek białek, w tym z wymianą rozpuszczalników i zatężaniem próbek.
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
Protokoły
Purification protocols for immunoglobulins generally include affinity binding to Protein A or G chromatography media, washing unbound proteins, and eluting with a low pH buffer.
Protokoły oczyszczania immunoglobulin zasadniczo obejmują wiązanie powinowactwa do podłoża chromatograficznego z białkiem A lub G, przemywanie niezwiązanych białek i elucję buforem o niskim pH.
Powiązane treści
Purification methods for downstream analysis reduce sample complexity, remove interferences, and concentrate analytes for more accurate results.
Metody oczyszczania do dalszej analizy zmniejszają złożoność próbki, usuwają zakłócenia i koncentrują anality w celu uzyskania dokładniejszych wyników.
Skoncentruj białko, nie trać go. Niezawodne odzyskiwanie białka podczas koncentrowania przy użyciu filtrów Amicon® Ultra.
Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.
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Can the product be washed and re-used?
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How many times can sample be added to the same filter unit? For example: If I have 100 mL cell free supernatant and want to add 12 mL at a time, centrifuge and collect retentate - followed by discarding the flow through and pouring the next 12 mL etc.
1 answer-
The same Amicon filter unit can be used for repeated spins with the same sample. However, repeated spins may lead to increased clogging, resulting in slower flow rates and longer spin times.
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I noticed no volume change after centrifuging 10mL cell media at 14,000xg for 20minutes. Could this be due to unsuccessful concentration?
1 answer-
The lack of volume change suggests that the sample may contain a high amount of particulate matter, leading to rapid clogging and preventing effective concentration.
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Hello! Our lab had to move to a different area of our building and we found some probably quite old boxes of Amicon Ultra 15s (Cat UFC903096, lot R8CN67048). Does this product have an expiration date/shelf life? If so, has this lot surpassed it?
1 answer-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment.
For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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