UFC5010
Amicon® Ultra Centrifugal Filter, 10 kDa MWCO
sample volume 0.5 mL, regenerated cellulose membrane, MWCO 10 kDa
Synonim(y):
Centrifugal concentrator, 10 kDa MWCO, 0.5 mL sample volume
About This Item
Polecane produkty
Materiały
polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing
Poziom jakości
sterylność
non-sterile
linia produktu
Amicon®
producent / nazwa handlowa
Amicon® Ultra
Parametry
0.5 mL sample volume
metody
protein extraction: suitable
protein purification: suitable
Długość
4.99 cm (1.96 in.)
Średnica
10.8 mm
powierzchnia filtracyjna
1 cm2
pojemność
0.5 mL
wielkość porów
10 kDa MWCO
Warunki transportu
ambient
Opis ogólny
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
Zastosowanie
- Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
- Desalting, buffer exchange, and protein dialysis
Cechy i korzyści
- Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 500 μL down to 15 μL
- Concentration factor 25x to 30x
- High sample recovery (greater than 90% of dilute starting solution)
- Fast processing time: 10 to 30 minutes
- Reverse spin capability provides consistent recoveries
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Produkty
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Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.
PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.
Filtration methods and membrane filter selection in nanoparticle purification and production.
Protokoły
Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.
If an antibody must be purified from a small sample volume (0.5-10 mL), then the Amicon® Pro Purification System is recommended.
Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.
Powiązane treści
Concentrate protein, don’t lose it. Recover protein reliably when you concentrate with Amicon® Ultra filters.
Skoncentruj białko, nie trać go. Niezawodne odzyskiwanie białka podczas koncentrowania przy użyciu filtrów Amicon® Ultra.
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
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