The low or no protein recovery issue may be attributed to over-concentration of the protein, leading to precipitation. The User Guide provides guidance on maximizing sample recovery: Low sample recovery in the concentrate may be due to adsorptive losses, over-concentration, or passage of sample through the membrane. Adsorptive losses depend upon solute concentration, its hydrophobic nature, temperature and time of contact with filter device surfaces, sample composition, and pH. To minimize losses, remove concentrated samples immediately after centrifugal spin. If the starting sample concentration is high, monitor the centrifugation process in order to avoid over-concentration of the sample. Over-concentration can lead to precipitation and potential sample loss. If the sample appears to be passing through the membrane, choose a lower MWCO Amicon device.
UFC8003
Amicon® Ultra Centrifugal Filter, 3 kDa MWCO
sample volume 4 mL, regenerated cellulose membrane, MWCO 3 kDa
Synonim(y):
Centrifugal concentrator, 3 kDa MWCO, 4 mL sample volume
Wybierz wielkość
Wybierz wielkość
About This Item
Polecane produkty
Materiały
polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
Poziom jakości
sterylność
non-sterile
linia produktu
Amicon®
opakowanie
pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg
producent / nazwa handlowa
Amicon® Ultra
sustainability
Greener Alternative Product
Parametry
4 mL sample volume
metody
protein extraction: suitable
protein purification: suitable
Długość
124 mm (4.88 in.)
Średnica
17.3 mm
powierzchnia filtracyjna
3 cm2
pojemność
4 mL
pojemność robocza
≤4 mL
4 mL
wielkość porów
3 kDa MWCO
kategoria ekologicznej alternatywy
Warunki transportu
ambient
Opis ogólny
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
Zastosowanie
- Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
Cechy i korzyści
- Ultracel® regenerated cellulose membrane with 3 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipette sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
Postać fizyczna
Informacje prawne
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Klienci oglądali również te produkty
Produkty
Filtration methods and membrane filter selection in nanoparticle purification and production.
Metody filtracji i wybór filtrów membranowych w oczyszczaniu i produkcji nanocząstek.
Poznaj zasady działania membran ultrafiltracyjnych i korzyści związane z przygotowywaniem próbek białek, w tym z wymianą rozpuszczalników i zatężaniem próbek.
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
Protokoły
The human plasma proteome is a vast resource for biomarker discovery and investigation.
Powiązane treści
Skoncentruj białko, nie trać go. Niezawodne odzyskiwanie białka podczas koncentrowania przy użyciu filtrów Amicon® Ultra.
-
What could be the reason for no protein recovery (in the concentrate or flow through) while using the UFC800324 to purify protein? Could the protein may be absorbed in the filter?
1 answer-
Helpful?
-
-
What is max g force these filters can take? i.e. what sort of rpm or g force should I spin at?
1 answer-
The UFC8003 protocol states to spin at 7,500 × g maximum for approximately 10–40 minutes. See the image below for further details.
Helpful?
-
-
Can we centrifuge these tubes in a normal 15 mL swinging bucket centrifuge?
1 answer-
Yes, the device can be used in centrifuges with a swinging bucket or fixed-angle rotor.
Helpful?
-
Active Filters
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej
