Alkaline Phosphatase (AP) catalyzes the hydrolysis of the phosphate group in alkaline media. AP is an essential enzyme and widely distributes in a variety of tissues such as liver, intestine, kidney, bone, and placenta. Abnormal AP levels have been shown in numerous cancers, bone disease and liver dysfunction. AP is also expressed by pluripotent stem cells such as ES and IPS cells.
The BioTracker CyP AP dye is a live cell near-infrared (NIR) fluorescent imaging probe for alkaline phosphatase. With its long emission wavelength, the probe is suitable for monitoring AP levels in both in vivo and in vitro tissues and cells. The probe possesses deep tissue penetration, less damage to the biological samples, and lower interference from the background fluorescence compared to other AP probes.
Store BioTracker CyP AP Live Cell Dye at -20°C, desiccate and protect from light Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Analysis Note
Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis Molar Mass: 528.55 g/mol
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Alkaline phosphatase (ALP) is an essential enzyme and widely distributes in a variety of tissues. To date, various nanomaterial and small-molecule fluorescent probes for ALP have been constructed successfully, but the emission wavelengths of these probes are in the ultraviolet
For how long can the cells be imaged after incubation with the reagent? Can the signal still be imaged following fixation and further immunofluorescence staining?
1 answer
Technical Support
·3 years ago
This product is for use with live cells, tissues, or animal models. As fixation effectively renders samples non-viable, no signal will be present in fixed tissues. The long term stability of the signal has not been established. The response arises and increases linearly with time, but may dissipate over time as well. Please see the link to review additional information: https://pubs.acs.org/doi/10.1021/acs.analchem.7b01351
