Yes, Product PKH67 would be suitable for this application. In this paper, both Products PKH26 and PKH67 are used in a similar application:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5679712/
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PKH67GL
PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling
Distributed for Phanos Technologies
Synonim(y):
Green PKH membrane labeling kit
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
1 kit | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 4380,00 zł |
Informacje o tej pozycji
NACRES:
NA.32
UNSPSC Code:
12352207
4380,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
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Pozwól nam pomócQuality Segment
packaging
pkg of 1 kit
storage condition
protect from light
fluorescence
λex 490 nm; λem 502 nm (PKH67 dye)
detection method
fluorometric
shipped in
ambient
storage temp.
room temp
Application
This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.
PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling has been used:
- To label and further investigate the exosomes expelled from cells in triple-negative breast cancer condition.
- To label apoptotic cells, in order to study the role of ανβ5 receptor in both binding and internalization of apoptotic cells.
- In fluorescence imaging.[1]
Other Notes
For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.
Legal Information
CellVue is a registered trademark of Phanos Technologies
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1 of 1
Ta pozycja | |||
|---|---|---|---|
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit |
| storage condition protect from light | storage condition protect from light | storage condition protect from light | storage condition protect from light |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
Tylko elementy zestawu
Numer produktu
Opis
- Diluent C 6 x 10
- PKH67 Cell Linker in ethanol .5 mL
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Klasa składowania
3 - Flammable liquids
flash_point_f
57.2 °F - closed cup
flash_point_c
14 °C - closed cup
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
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I mark mitochondria for injection with PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling. I want to use another dye to mark another type of mitochondria and inject them together in the same fish egg. Is PKH67 ok for this like PKH26?
1 answer-
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What can be the alternative for removing excess dye after labeling of exosomes using PKH67 dye apart from ultracentrifugation?
1 answer-
Excess dye is bound to serum or albumin which is added to the cell/dye mixture as a stop reaction. The recommended clean up or dye removal is by standard centrifugation (400 x g for 10 minutes, wash, and repeat). See the Product Information Sheet, page 3, bullet point 8 for more information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/984/984/pkh67glbul.pdfAlternate methods for excess dye removal have not been investigated.
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Can we remove the excess PKH67 DYE used for the labeling of exosomes using exosome spin column rather than ultracentrifugation.
1 answer-
The recommended exosome labeling protocol has been optimized to assure the best possible results. The use of exosome spin columns with the PKH and CellVue products has not been validated. See below for a link to our exosome labeling protocol. A spin column method may not offer the best results.
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What method of fixation can be used for tissue/cells with the PKH Fluorescent Cell Linker Kit for General Cell Membrane Labeling dyes?
1 answer-
A protocol for visualization of tissue sections can be found in the technical bulletin. For visualization of stained cells by immunofluorescence or flow cytometry, the cells can be fixed in 2% paraformaldehyde for 15 minutes. The use of other organic solvents will extract the dye from the cells. If internal labeling is desired, the cells can be permeabilized with saponin (50-75 μg/mL). Here is the link to the Troubleshooting Guide.
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Are cells lost during the PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling staining process?
1 answer-
Over-labeling of the cells will result in loss of membrane integrity and reduced cell recovery. Methods for improving cell viability can be found on the troubleshooting guide.
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Does the dye leak from the cells after labeling when using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling?
1 answer-
In general, slow loss of fluorescence has been observed with PKH1 and PKH2 in in vivo studies and it is likely that PKH67 may also slowly lose fluorescence. Leakage (or loss of staining intensity) may also occur due to cell-to-cell transfer of the dye. The most common cause of cell-to-cell dye transfer is inadequate washing of the cells. Wash cells 3-5 times after labeling and transfer samples to new tubes between washes.
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What is the excitation and emission spectra for Product PKH67GL, Green Fluorescent Cell Linker Kit?
1 answer-
The spectra can be found on the product insert.The product has a maximum excitation at 490 nm and maximum emission at 502 nm.
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How many cells can be stained with Product PKH67GL, Green Fluorescent Cell Linker Kit?
1 answer-
This kit can stain 50 × 107 cells if used as directed (1 × 107 cells stained with 2 × 10-6 M PKH67).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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When using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling, how long will the cells remain stained after treatment?
1 answer-
Labeled cells that have been washed can be visualized in culture up to 100 days after staining (for non-dividing cells). The dye itself is stable and will divide equally when the cells divide. After staining with PKH dyes, you can observe as many as 8 divisions depending on how brightly the cells were stained initially and the amount of surface area on the cells. Most commonly, 4-6 divisions can be visualized.
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