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SCM141

Sigma-Aldrich

PLTMax Human Platelet Lysate

PLTMax Human Platelet Lysate is a growth factor rich supplement that is a superior alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) culture. 100ml.

Synonim(y):

hPL, hPL Suplement lizatu ludzkich płytek krwi

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100 ML
2000,00 zł

2000,00 zł


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Wybierz wielkość

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100 ML
2000,00 zł

About This Item

Kod UNSPSC:
12352207
eCl@ss:
32160801
NACRES:
NA.75

2000,00 zł


Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

pochodzenie biologiczne

human

Poziom jakości

Formularz

liquid

producent / nazwa handlowa

Chemicon®

metody

cell culture | stem cell: suitable

moc wejściowa

sample type mesenchymal stem cell(s)

Warunki transportu

dry ice

Opis ogólny

PLTMax Human Platelet Lysate is a growth factor rich supplement that is a superior alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) culture. Optimal growth of MSCs can be achieved with 5% PLTMax Human Platelet Lysate. PLTMax Human Platelet Lysate has been used to effectively to grow mesenchymal stem cells derived from other species including mouse, rabbit and porcine.

PLTMax Human Platelet Lysate is derived from normal human donor platelets collected at U.S. blood centers. Multiple donors units are pooled in large batch sizes and manufactured to produce a consistent product. Heparin (porcine) approved for human intravenous use is used in the preparation of PLTMax Human Platelet Lysate. This product contains human derived materials and must be treated as potentially infectious. Universal precautions for biological samples should be used in handling and disposal.

Zastosowanie

Instructions for Use
• Add Heparin to the media at a final concentration of 2 U/mL. Failure to add heparin may result in coagulation of certain media.
• For optimal growth of human mesenchymal stem cells, add 5% vol/vol PLTMax Human Platelet Lysate to usual cell culture medium such as DMEM and αMEM.
• Plate cells at approximately 1.5x103 cells per cm2.
• Do not allow primary MSC confluence to exceed 80%.

Particulate Formation
• Product has been aseptically filtered with 0.2 µm filters. Particulates will be observed after thawing. Particulate formation is normal will not affect performance even in the presence of large particulates. Filtration is not recommended.
• To reduce particulates from forming, minimize freeze/thaw cycles and extended storage at 4°C. Some particulate formation can be reversed with brief incubation at 37°C, if desired.
PLTMax Human Platelet Lysate is a growth factor rich supplement that is a superior alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) culture. 100ml.
Research Category
Stem Cell Research
Research Sub Category
Mesenchymal Stem Cells

Jakość

PLTMax Human Platelet Lysate is manufactured with the highest quality of raw materials. All human derived materials are obtained from government inspected facilities and are of US origin. Each donor unit was approved for human use and has been tested for infectous diseases including HIV-1, HIV-2, HCV, HBsAg, and RPR for Syphilis.

Each lot is extensively tested for the following parameters:
pH: 6.8-7.8
Total protein: 4.0-6.5 g/dl
Endotoxin USP <8>: <10 EU/mL
Mycoplasma: Not detected
Sterility Testing: Negative for growth

Przechowywanie i stabilność

Store at -80°C upon receipt. Thaw at room temperature or in a water bath. After thawing, aliquot and store at -20°C or colder. Long term storage at -80°C is recommended. Multiple freeze/thaw cycles are not recommended.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1


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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Samatha Bhat et al.
Scientific reports, 11(1), 3403-3403 (2021-02-11)
Bone marrow-derived mesenchymal stromal cells (BM-MSCs) are gaining increasing importance in the field of regenerative medicine. Although therapeutic value of MSCs is now being established through many clinical trials, issues have been raised regarding their expansion as per regulatory guidelines.
Adrian Gericke et al.
RSC advances, 9(39), 22531-22539 (2019-07-22)
Impaired regeneration of the corneal epithelium, as found in many ocular surface diseases, is a major clinical problem in ophthalmology. We hypothesized that corneal epithelial regeneration can be promoted by the physiological, energy-delivering as well as "morphogenetically active" polymer, inorganic
Mark Li et al.
Stem cells translational medicine, 10(3), 385-397 (2020-10-25)
To optimize the regenerative proficiency of stem cells, a cardiopoietic protein-based cocktail consisting of multiple growth factors has been developed and advanced into clinical trials for treatment of ischemic heart failure. Streamlining the inductors of cardiopoiesis would address the resource
Augustin M Ofiteru et al.
Cells, 9(12) (2020-12-03)
Potency assays are critical for regenerative medicine, addressing the known challenge of functional heterogeneity among human multipotent stromal cells (hMSC). Necessary laboratory cell expansion allows analysis before implantation in the patient. Levels of induction of five signature gene biomarkers, ALPL

Produkty

Highly characterized human mesenchymal stem cell lines from bone-marrow, adipose and umbilical cord along with serum-free MSC expansion and differentiation media.

Zapoznaj się z rodzajami komórek macierzystych, w tym multipotencjalnymi komórkami macierzystymi, pluripotencjalnymi komórkami macierzystymi i iPSC oraz ich zastosowaniami w podstawowych badaniach nad komórkami macierzystymi, terapii komórkami macierzystymi i modelowaniu chorób.

Read types of stem cells including multipotent stem cells, Pluripotent stem cells and iPSCs and their applications in basic stem cell research, stem cell therapy and disease modelling

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

Zobacz wszystko

Protokoły

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

Informacje na temat mezenchymy, w szczególności protokołów mezenchymalnych komórek macierzystych. Protokoły hodowli komórkowej krok po kroku do izolacji, ekspansji i różnicowania mezenchymalnych komórek macierzystych (MSC).

Questions

  1. GMP/CLINICAL GRADE로 개발이 계획되어 있는지 문의드립니다.

    1 answer
    1. This product is not scheduled to be or listed as a GMP grade or clinical grade product. This product falls under a Quality Level 100, products with a quality level 100 are not suitable for GMP use.

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