MABS2000

Anti-LXR alpha Antibody, clone 2B7

clone 2B7, from mouse

• Synonim(y): Oxysterols receptor LXR-alpha, Liver X receptor alpha, Nuclear receptor subfamily 1 group H member 3, RLD-1
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 2B7, monoclonal
• reaktywność gatunkowa: mouse, rat
• opakowanie: antibody small pack of 25 μg
• metody: immunoprecipitation (IP): suitable; western blot: suitable
• izotyp: IgG1κ
• numer dostępu UniProt: Q62685
• Warunki transportu: ambient
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: mouse ... Nr1H3(22259)

Opis

Opis ogólny

Oxysterols receptor LXR-alpha (UniProt: Q62685; also known as Liver X receptor alpha, Nuclear receptor subfamily 1 group H member 3, RLD-1) is encoded by the Nr1h3 (also known as Lxra) gene in rat. Liver X receptors (LXRs) are members of the nuclear receptor superfamily that have been implicated in lipid homeostasis and glucose metabolism. Oxysterols, oxidized derivatives of cholesterol, are ligands for LXRs. LXRs act as cholesterol sensors; when cellular oxysterols accumulate as a result of increasing concentrations of cholesterol, LXR induces the transcription of genes that protect cells from cholesterol overload. LXRs bind to consensus elements (LXR response elements, LXREs) as heterodimers with isoforms of the retinoid X receptor (RXR). LXR-alpha is an important regulator of cholesterol and bile acid metabolism. It interacts with RXR and shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. In adults it is expressed in spleen, pituitary, lung, liver, and fat and a weaker expression is observed in several other tissues. LXR-alpha contains a transactivation domain (AF-1; aa 1-94) that is required for ligand-independent transactivation function; another transactivation domain (AF-2; aa 203-445) that is required for ligand-dependent transactivation function; and a ligand binding domain (aa 213-432). (Ref.: Zhao, C., and Dahlman-Wright, K. (2010). J. Endocrinol. 204, 233-240).

Specyficzność

Clone 2B7 detects LXR-alpha in mouse and rat liver tissue.

Immunogen

GST-tagged full length recombinant rat LXR-alpha protein.

Zastosowanie

Anti-LXR alpha, clone 2B7, Cat. No. MABS2000, is a mouse monocloanl antibody that detects Oxysterols receptor LXR-alpha and has been tested for use in Immunoprecipitation and Western Blotting.

Immunoprecipitation Analysis: A representative lot detected LXR alpha in Immunoprecipitation applications (Tian, J., et. al. (2016). Proc Natl Acad Sci USA. 113(29):8182-7).

Western Blotting Analysis: A representative lot detected LXR alpha in Western Blotting applications (Tian, J., et. al. (2016). Proc Natl Acad Sci USA. 113(29):8182-7).

Research Category
Signaling

Jakość

Evaluated by Western Blotting in mouse liver tissue lysate.

Western Blotting Analysis: 1 ug/mL of this antibody detected LXR alpha in 10 µg of mouse liver tissue lysate.

Opis wartości docelowych

~50 kDa observed; 50.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Postać fizyczna

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable