MABS1217

Anti-Fibulin-1 Antibody, clone 3D12.1

clone 3D12.1, from mouse

• Synonim(y): FIBL-1, Fibulin-1
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 3D12.1, monoclonal
• reaktywność gatunkowa: human
• metody: immunohistochemistry: suitable; western blot: suitable
• izotyp: IgG2aκ
• numer dostępu NCBI: NP_001987
• numer dostępu UniProt: P23142
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... FBLN1(2192)

Opis

Opis ogólny

Fibulin-1 (UniProt P23142; also known as FIBL-1) is encoded by the FBLN1 (also known as FIBL1, FBLN, PP213) gene (Gene ID 2192) in human. Fibulin-1 is an extracellular matrix protein that interacts with fibronectin and inhibits cell migration and invasion in a fibronectin-dependent manner. Research shows that the EGF-like domains 5 and 6 of fibulin-1 compete against syndecan-4 for binding the C-terminal heparin-binding domain (HepII) of fibronectin, resulting in a suppression of fibronectin-regulated cell adhesion and motility. Fibulin-1 also interacts with fibrinogen and regulates platelet adhesion to influence hemoglutination. In addition, fibulin-1 interacts with elastin and plays a role in the assembly of elastic fibers by mediating association between versican and fibrillin. Other fibulin-1 interactors include calumenin, extracellular matrix protein 1, angiogenin, and hemicentin. There exist four major fibulin-1 isoforms (A, B, C, and D) generated by alternative splicing, with isoforms C and D being the predominant forms. In addition, a smaller isoform of fibulin-1D, fibulin-1D′, is reported to localize in the cytosol and interacts with the intracellular domain of integrin β1. Fibulin-1 is initially produced with a signal peptide (a.a. 1-29), the removal of which yields the mature protein with three N-terminal Anaphylatoxin-like domains (a.a. 36-76, 77-111, 112-144), followed by eight EGF-like domains (a.a. 176-578 of isoform D), with EGF-like domains 5 & 6 mediating fibulin-1 self-association and fibronectin-binding. The C-terminal 137-amino acid in isoform D is truncated in isoform A, and replaced by a shorter sequence of 35 amino acids in isoform B and a sequence of 117 amino acids in isoform C.

Specyficzność

Expected to react with all four spliced isoforms of human fibulin-1 (UniProt P23142-1 to P23142-4).

Immunogen

Epitope: EGF-like domains 4-6.

GST-tagged recombinant protein corresponding to EGF-like domains 4-6 of human Fibulin-1.

Zastosowanie

Detect Fibulin-1 using this Anti-Fibulin-1 Antibody, clone 3D12.1 validated for use in Western Blotting, Immunohistochemistry.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Fibulin-1 in human placenta and human breast tissue.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Jakość

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: 4.0 µg/mL of this antibody detected Fibulin-1 in 10 µg of SH-SY5Y cell lysate.

Opis wartości docelowych

~77 kDa observed. 77.2 kDa (isoform D), 61.6 kDa (isoform A), 65.4 kDa (isoform B), and 74.4 kDa (Isoform C) calculated.

Postać fizyczna

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable