MAB3083
Anti-Calpain Antibody, small subunit of µ- or m-Calpains (Calpain I or II), clone P1
ascites fluid, clone P1, Chemicon®
Synonim(y):
Anti-CALPAIN4, Anti-CANP, Anti-CANPS, Anti-CAPN4, Anti-CDPS, Anti-CSS1
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Informacje o tej pozycji
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
P1, monoclonal
Application:
ELISA, ICC, IHC, WB
Citations:
12
Pomoc techniczna
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Pozwól nam pomócbiological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
P1, monoclonal
species reactivity
human, bovine
should not react with
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... CAPNS1(826)
General description
Calpains are non-lysosomal cysteine proteeases which absolutely require calcium for activity. There are two major calpain isozymes: μ-calpain and m-calpain, which require micromolar or millimolar calicum for activity, respectively, in invitro assays. The major calpain isozymes are composed of two subunits: an 80kDa catalytic subunit; and a 30kDa regulatory subunit. The large and small subunits remain associated in the presence of calcium after proteolytic activation of the zymogen.
Immunogen
Bovine myocardial m-calpain.
Epitope: small subunit of μ- or m-Calpains (Calpain I or II)
Application
Immunoblot: 1:500-1,000. The small subunit of either μ-calpain m-calpain is 30kDa. ELISA: 1:50,000 (against the immunogen). Immunohistochemistry: 1:100 on 4% paraformaldehyde or 1% paraformaldehyde/4% glutaraldehyde fixed tissue. Immunocytochemistry: 1:100. Optimal working dilutions must be determined by the end user.
This Anti-Calpain Antibody, small subunit of μ-or m-Calpains (Calpain I or II), clone P1 is validated for use in ELISA, WB, IC, IH for the detection of Calpain.
Biochem/physiol Actions
Reacts with the small subunit of human erthrocyte μ-calpain (Calpain I) or bovine myocardial m-calpain (Calpain II).
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Klasa składowania
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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