Typically, a minimum of 3-6 amino acids is used to generate an antibody. Peptides that are 10-20 amino acids long are considered ideal for antibody preparation, while those used for structure/function studies can vary in length. In the literature, the N-terminal fragments of Huntingtin in the cortex have been fully characterized. The second protease-susceptible domain, known as the “B” domain, is expected to be located farther from the N-terminal and in proximity to the region corresponding to residues 181–810. This sequence is utilized to validate the specificity of the antibody.
MAB2166
Anti-Huntingtin Disease (HD/HTT) Antibody
CHEMICON®, mouse monoclonal, 1HU-4C8
Synonim(y):
Huntingtin, Huntington′s Disease Protein, HD Protein
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 100 μL | Przewidywany termin wysyłki11 maja 2026zKuehne + Nagel Sp. z o.o. | 2630,00 zł |
Informacje o tej pozycji
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
1HU-4C8, monoclonal
Species reactivity:
rat, rabbit
Application:
ELISA, ICC, IHC, IP, WB
Citations:
327
2630,00 zł
Przewidywany termin wysyłki11 maja 2026Szczegóły
Rekombinowane, niezawierające konserwantów przeciwciało jest dostępne dla Twojego celu. Wypróbuj ZRB1102
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Pozwól nam pomócNazwa produktu
Anti-Huntingtin Protein Antibody, a.a. 181-810, clone 1HU-4C8, ascites fluid, clone 1HU-4C8, Chemicon®
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
1HU-4C8, monoclonal
species reactivity
rat, rabbit
species reactivity (predicted by homology)
mouse, human, hamster, monkey
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... HTT(3064), SLC6A4(6532)
General description
Huntington disease (HD) is a hereditary, progressive, neurodegenerative ailment characterized by personality changes, motor impairment and subcortical dementia. The molecular basis of the disease involves the expansion of the trinucleotide CAG, coding for polyglutamine in the first exon of a chromosome four gene (4p16.3), which normally produces a widely expressed 3136 a.a. (~350 kDa) protein huntingtin with unclear function. The protein is found in the perinuclear region along with microtubules, and in the centrosomal region along with gamma-tubulin. Huntingtin is necessary for neuronal survival and is involved in synaptic vesicle trafficking, microtubule binding and may also have a role in apoptosis. In the HD condition, neuronal cells with the mutant form of huntingtin possess intranuclear aggregations of the N-terminal fragment, causing damaging inclusions in perinuclear locations and striatal neuron cell death. Wild-type huntington and anti-huntingtin reduce aggregation and cellular toxicity of the mutant huntingtin form in mammalian cell models of HD. Huntingtin is known to interact with GAPDH, HAP-1, SP1 and TAFII130.
~ 350-400 kDa
Immunogen
Epitope: a.a. 181-810
Huntingtin fragment from a.a. 181 to 810 as a fusion protein.
Application
Anti-Huntingtin Protein Antibody, a.a. 181-810, clone 1HU-4C8 is an antibody against Huntingtin Protein for use in ELISA, IC, IH(P), IP & WB.
ELISA:
A 1:500-1:5,000 dilution of a previous lot was used on ELISA.
Immunohistochemistry:
A 1:500-1:5,000 dilution from a previous lot was used on frozen and microwave oven treated paraffin sections (human tissue).
Immunocytochemistry:
1:500-1:5,000 on a previous lot was used on transfected cells.
Immunoprecipitation:
A 1:500-1:5,000 dilution of a previous lot was used on immunoprecipitation.
Western blot:
1:500-1:5,000. Should detect a band migrating at approximately 350-400 kDa by Western blot (Nature Genetics 10:104-110.).
Optimal working dilutions must be determined by the end user.
A 1:500-1:5,000 dilution of a previous lot was used on ELISA.
Immunohistochemistry:
A 1:500-1:5,000 dilution from a previous lot was used on frozen and microwave oven treated paraffin sections (human tissue).
Immunocytochemistry:
1:500-1:5,000 on a previous lot was used on transfected cells.
Immunoprecipitation:
A 1:500-1:5,000 dilution of a previous lot was used on immunoprecipitation.
Western blot:
1:500-1:5,000. Should detect a band migrating at approximately 350-400 kDa by Western blot (Nature Genetics 10:104-110.).
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
Biochem/physiol Actions
Huntingtin Protein. No detectable cross reactivity to other proteins by Western blot.
Physical form
Ascites mouse monoclonal IgG1κ liquid containing no preservative
Unpurified
Preparation Note
Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Normal human cerebral cortex lysate
Normal human cerebral cortex lysate
Routinely evaluated by Western Blot on rat brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected huntingtin protein on 10 μg of rat brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected huntingtin protein on 10 μg of rat brain lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Ta pozycja | |||
|---|---|---|---|
| Gene Information human ... HTT(3064), SLC6A4(6532) | Gene Information human ... HTT(3064), SLC6A4(6532) | Gene Information human ... HTT(3064), SLC6A4(6532) | Gene Information human ... HTT(3064) |
| biological source mouse | biological source mouse | biological source mouse | biological source rabbit |
| clone 1HU-4C8, monoclonal | clone mEM48, monoclonal | clone HU-2E8, monoclonal | clone polyclonal |
| species reactivity rat, rabbit | species reactivity human | species reactivity monkey, human | species reactivity mouse, human |
| antibody form ascites fluid | antibody form culture supernatant | antibody form ascites fluid | antibody form affinity isolated antibody |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
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Klasa składowania
12 - Non Combustible Liquids
wgk
WGK 2
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Protokoły
Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.
Produkty
Immunofluorescencja wykorzystuje cząsteczki fluorescencyjne sprzężone z przeciwciałami do lokalizacji białek, potwierdzania modyfikacji i wizualizacji kompleksów białkowych.
Immunofluorescence uses antibody-conjugated fluorescent molecules for protein localization, modification confirmation, and protein complex visualization.
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Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| MAB2166 | 04053252396847 |
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Is the epitope binding site for the MAB2166 antibody to Huntingtin, spanning AA 181 to 810, considered a wide range for binding due to the protein's confirmation that enables the antibody to bind these two sites?
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