IHCR1002-6

Anti-Beta (β)-Amyloid antibody

CHEMICON^®, mouse monoclonal, 22C11

• Synonim(y): APP
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• Nazwa produktu: Anti-APP A4 Antibody, a.a. 66-81 (NT), Prediluted, clone 22C11, clone 22C11, Chemicon^®, from mouse
• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• rodzaj przeciwciała: primary antibodies
• klon: 22C11, monoclonal
• reaktywność gatunkowa: canine, mouse, monkey, pig, fish, human, rat
• producent / nazwa handlowa: Chemicon^®
• metody: immunohistochemistry: suitable (paraffin)
• izotyp: IgG1
• numer dostępu NCBI: NM_000484.2; NM_201413.1; NM_201414.1
• numer dostępu UniProt: P05067
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... APP(351)

Opis

Opis ogólny

Deposits of amyloid protein in senile plaques near nerve processes are found in the brains of aged humand and cases of Alzheimer′s Disease. The principle component of this extracellular amyloid is beta/A4, a 4 kDa peptide derived from a larger amyloid precursor protein (APP), which is widely expressed in the brain and body. The creation, transport and function of these proteins are currently under investigation Reacts with pre-A4.

Specyficzność

The antibody recognizes amino acids 66-81 of the N-terminus on the pre-A4 molecule (Hilbich et al., 1993). 22C11 recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa (Hoffmann et al., 2000). The antibody is known to cross react with APLP2 (Slunt, 1994).

Immunogen

Epitope: a.a. 66-81 (N-terminus), Prediluted

Purified recombinant Alzheimer precursor A4 (pre A4695) fusion protein.

Zastosowanie

Antibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.

Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.

Incubation: 30 minutes with IHC Select Detection Kits.

App has been prediluted for use as the primary antibody with Chemicon′s IHC Select Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier′s IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No. IHCR1002-6.

Detect Alzheimer Precursor Protein A4 using this Anti-APP A4 Antibody, a.a. 66-81 (N-terminus), Prediluted, clone 22C11 validated for use in IH(P).

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Postać fizyczna

Format: Purified

Liquid diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% Kathon as preservative.

Przechowywanie i stabilność

Maintain at 2-8°C. Refer to vial for expiration dating.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H317,H412
• Zwroty wskazujące środki ostrożności: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Klasyfikacja zagrożeń: Aquatic Chronic 3 - Skin Sens. 1
• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable