AB5168
Anti-CHAPSYN-110 Antibody
Chemicon®, from rabbit
Synonim(y):
PSD-93, Discs Large Homolog 2, DLG2
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
polyclonal
reaktywność gatunkowa
rat
producent / nazwa handlowa
Chemicon®
metody
immunohistochemistry: suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
rat ... Dlg2(64053)
Specyficzność
Recognizes a full length chapsyn-110 protein. It has exhibited no cross reactivity with other known related proteins tested so far.
The immunogen sequence is 42 of 44 amino acids identical in human.
Immunogen
GST fusion protein (Smith & Johnson 1988) and of rat chapsyn-110 (amino acids 336-379) (Brenman et al. 1996; Kim et al. 1996).
Zastosowanie
This Anti-CHAPSYN-110 Antibody is validated for use in WB, IH for the detection of CHAPSYN-110.
Western blot: 1 to 3 μg/mL (1:300-1:1000) using ECL
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Postać fizyczna
Format: Purified
Purified immunoglobulin by Protein A. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Komentarz do analizy
Control
CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 200 μL of sterile deionized water. For positive control, in Western blot using 10 ng of protein per lane. For negative control, preincubate 1 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 200 μL of sterile deionized water. For positive control, in Western blot using 10 ng of protein per lane. For negative control, preincubate 1 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Certyfikaty analizy (CoA)
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