539134
Protease Inhibitor Cocktail III
EDTA-Free, liquid, for the inhibition of aspartic, cysteine, and serine proteases and aminopeptidases in mammalian cells and tissues.
Synonim(y):
EDTA free Protease inhibitor, EDTA-Free Protease Inhibitor Mix, Protease inhibitor cocktail III
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About This Item
Kod UNSPSC:
12352200
NACRES:
NA.77
Formularz:
liquid
temp. przechowywania:
−20°C
Informacje o cenach i dostępności nie są obecnie dostępne.
Polecane produkty
Nazwa produktu
Protease Inhibitor Cocktail Set III, EDTA-Free, Protease inhibitor cocktail III, EDTA-free for inhibiting aspartic, cysteine, and serine proteases as well as aminopeptidases in mammalian cells and tissues.
Formularz
liquid
producent / nazwa handlowa
Calbiochem®
warunki przechowywania
OK to freeze
Warunki transportu
wet ice
temp. przechowywania
−20°C
Powiązane kategorie
Opis ogólny
Note: available as 1 x 1 ml or as a set of 5 x 1 ml.
This protease inhibitor cocktail set is a specially formulated cocktail of six protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases. This cocktail is recommended for use with mammalian cells and tissue extracts, but is also suitable for bacterial extracts. It is provided as a single vial or as a set of 5 vials. Each vial contains 1 ml protease inhibitor cocktail solubilized in DMSO with a small amount of water.
Specyficzność
Inhibits a broad spectrum of serine proteases, cysteine proteases, and aspartic proteases, as well as aminopeptidases.
Zastosowanie
Protease Inhibitor Cocktail Set III, EDTA-Free has been used:
- as a component in CelLytic MT cell lysis reagent or lysis buffer to homogenize kidney organoids for immunoblot analysis[1]
- as a component in homogenization buffer to homogenize minced mouse quadriceps femoris muscle tissue[2]
- in lysis buffer to extract proteins for immunoblotting[3]
Działania biochem./fizjol.
Protease inhibitor cocktails and specialized protease inhibitors play a crucial role in various proteomic studies conducted by scientists. These studies encompass protein expression and characterization, biomarker discovery, identification of post-translational modifications, protein expression profiling, and quantitative protein measurement. The inclusion of a protease inhibitor cocktail or specific protease inhibitor guarantees the preservation of protein integrity, allowing for accurate downstream analysis and comprehensive sample characterization.
Cechy i korzyści
- EDTA-Free: recommended for IMAC applications.
- Flexible: compatible with bacterial extracts, mammalian cell extracts, and tissue extracts.
- Fast: no waiting time for tablets to dissolve.
- Economical: ready-to-use format reduces wastage.
- Effective: one ml is sufficient for approximately 20g of tissue.
- Complete: contains six protease inhibitors to inhibit aspartic, cysteine, serine proteases, and aminopeptidases.
Ostrzeżenie
Toxicity: Irritant (B)
Rekonstytucja
Following initial thaw, aliquot and freeze (-20°C).
Informacje prawne
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Irrit. 2 - Skin Irrit. 2
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
188.6 °F - (refers to pure substance)
Temperatura zapłonu (°C)
87 °C - (refers to pure substance)
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Produkty
Learn tips and tricks for how to properly use inhibitors including how to select the right inhibitor and how to plan experiments with inhibitors.
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