529659

DDD00033325

≥95% (HPLC), syrup, single hESC survival promoter, Calbiochem^®

• Synonim(y): Pro-survival Compound, DDD00033325, DDD00033325
• Wzór empiryczny (zapis Hilla): C₁₇H₁₉N₅
• Numer CAS: 1259034-82-6
• Masa cząsteczkowa: 293.37
• Kod UNSPSC: 51111800
• NACRES: NA.77

Właściwości

• product name: Pro-survival Compound, DDD00033325, A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background with equal or higher potency than Y-27632.
• Poziom jakości: 100
• Próba: ≥95% (HPLC)
• Postać: syrup
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; protect from light
• kolor: yellow
• rozpuszczalność: DMSO: 100 mg/mL
• Warunki transportu: wet ice
• temp. przechowywania: −20°C
• ciąg SMILES: C12=CC=NN1C=CC(N[C@@H]3CCN(CC4=CC=CC=C4)C3)=N2

Opis

Opis ogólny

A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background (EC₅₀ = 3.9 M), with equal or higher potency than Y-27632 (Cat. No. 688000) in 5 cell lines (SA461, SA181, SA167, SA121 and SA002) tested at 10 M. An in vitro high-content screening assay, which profiles a panel of more than 70 protein kinases, indicates that this small molecule is more selective than Y-27632 and only displays an inhibitory effect toward ROCK2 (>83% inhibition, ROCK1 was not included in the panel) and PRK2 (76% inhibition) at 10 M. Furthermore, it exhibits less cytotoxicity at a greater concentration of 30 M than Y-27632 without marked adverse effects for up to 120 hours.

A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background (EC₅₀ = 3.9 M), with equal or higher potency than Y-27632 in 5 cell lines (SA461, SA181, SA167, SA121 and SA002) tested at 10 M. An in vitro high-content screening assay, which profiles a panel of more than 70 protein kinases, indicates that this small molecule is more selective than Y-27632 and only displays an inhibitory effect toward ROCK2 (>83% inhibition, ROCK1 was not included in the panel) and PRK2 (76% inhibition) at 10 M. Furthermore, it exhibits less cytotoxicity at a greater concentration of 30 M than Y-27632 without marked adverse effects for up to 120 hours.

Opakowanie

Packaged under inert gas

Ostrzeżenie

Toxicity: Regulatory Review (Z)

Rekonstytucja

Following reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.

Inne uwagi

Andrews, P.D., et al. 2010. Biochem. J.432, 21.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable