17-10520

Magna 4699 RIP (usieciowany) zestaw do immunoprecypitacji jądrowego białka wiążącego RNA

• Kod UNSPSC: 41105331
• NACRES: NA.84

Właściwości

• Poziom jakości: 100

Opis

Opis ogólny

Magna Nuclear RIP Kits are specially designed to allow the discovery and analysis of a variety of chromatin associated RNAs such as long non-coding RNAs, enhancer RNAs and miRNAs . These chromatin-associated RNAs often regulate gene expression and can be analyzed with applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and next generation sequencing (RIP-Seq).

Nuclear RIP can be performed using chromatin that has interactions stabilized by formaldehyde treatment (cross-linked) or chromatin that has not been treated with a cross linking reagent (native). While both of these approaches are similar in that they are designed to recover chromatin associated RNA, the reagents used and the details of the protocol and types of interactions typically detected are different. Cross-linked can capture higher molecular weight complexes in in vivo configurations with possibly lower affinities. In contrast native RIP is expected to recover high affinity, more direct interactions between proteins encoded RNA binding motifs and candidate RNAs. For less well understood proteins and protein complexes often both approaches are used.

Zastosowanie

Magna Nuclear RIP (Cross-Linked) RNA-Binding Protein Immunoprecipitation Kit is designed for the analysis of chromatin associated RNA such lncRNAs, enhancer RNAs and miRNAs.

Opakowanie

Kit capacity: 24 RNA-binding protein immunoprecipitation assays using a cross-linked nuclear lysate

Komponenty

10X Glycine;10X PBS;Nuclei Isolation Buffer;RIP Cross-Linked Lysis Buffer;Protein A/G Magnetic Beads;Nuclear RIP Dilution Buffer;Low Salt Wash Buffer;High Salt Wash Buffer;LiCl Wash Buffer;TE Buffer;RIP Elution Buffer;10% SDS;0.5 M EDTA;DNase I (RNase Free);DNase I Supplement;DNase I Reaction Buffer ;Protease Inhibitor Cocktail III, Animal Free ;RNAse Inhibitor;Proteinase K

Informacje prawne

NuCLEAR is a trademark of Sigma-Aldrich Co. LLC

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS05,GHS09
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H315,H318,H410
• Zwroty wskazujące środki ostrożności: P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Klasyfikacja zagrożeń: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
• Kod klasy składowania: 10 - Combustible liquids