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777323

Cy5-azide

95% (HPLC)

Synonim(y):

Cy5 Azide Fluorescent Probe

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Rozmiar/SKUDostępnośćCena netto
1 mg
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728,00 zł
5 mg
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2020,00 zł

Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):
C49H78N8O10S3
Masa cząsteczkowa:
1035.39
UNSPSC Code:
12352125
PubChem Substance ID:
NACRES:
NA.22
Assay:
95% (HPLC)
Form:
solid

728,00 zł


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Quality Segment

assay

95% (HPLC)

form

solid

reaction suitability

reaction type: click chemistry

ε (extinction coefficient)

251,000

fluorescence

λex 647 nm; λem 663 nm

storage temp.

−20°C

SMILES string

CC1(C)C(/C=C/C=C/C=C(N2CCCS([O-])(=O)=O)\C(C)(C)C3=C2C=CC(S([O-])(=O)=O)=C3)=[N+](CCCCCC(NCCCN=[N+]=[N-])=O)C4=C1C=C(S([O-])(=O)=O)C=C4.CC[NH+](CC)CC.CC[NH+](CC)CC

InChI

1S/C37H48N6O10S3.2C6H15N/c1-36(2)29-25-27(55(48,49)50)16-18-31(29)42(22-10-6-9-15-35(44)39-20-11-21-40-41-38)33(36)13-7-5-8-14-34-37(3,4)30-26-28(56(51,52)53)17-19-32(30)43(34)23-12-24-54(45,46)47;2*1-4-7(5-2)6-3/h5,7-8,13-14,16-19,25-26H,6,9-12,15,20-24H2,1-4H3,(H3-,39,44,45,46,47,48,49,50,51,52,53);2*4-6H2,1-3H3

InChI key

NJSWGOLBDDXJQO-UHFFFAOYSA-N

Application

Azide modified cyanine dye that can be used to detect or label alkyne- or cyclooctyne-containing molecules or biomolecules by fluorescence spectroscopy following azide-alkyne cycloaddition.

Solubility: DMSO, DMF, MeOH
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Ta pozycja
777358C1117MAB505S
description

95% (HPLC)

description

95% (HPLC)

description

clone CY5-15, purified from hybridoma cell culture

description

Chemicon®, from rat

assay

95% (HPLC)

assay

95% (HPLC)

assay

-

assay

-

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

100

reaction suitability

reaction type: click chemistry

reaction suitability

-

reaction suitability

-

reaction suitability

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

form

solid

form

powder

form

buffered aqueous solution

form

-

fluorescence

λex 647 nm; λem 663 nm

fluorescence

-

fluorescence

-

fluorescence

-


Piktogramy

Exclamation mark

Słowo ostrzegawcze

Warning

Kody zagrożeń

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Narządy docelowe

Respiratory system

Klasa składowania

11 - Combustible Solids

Co się dzieje?

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable



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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Hi, I am trying to conjugate cy5 to a protein already has azide at its C-terminus. What would the protocol would be in this case? Do we need to use CuAAC in our case?

    1 answer
    1. This product is a Cy5-azide. Conjugation may not be possible if the protein of interest has a terminal azide rather than a terminal alkyne. Should this be the case, a Cy5-alkyne such as product 777358, would be recommended. Click chemistry relies on 1,3-dipolar cycloaddition of alkynes to azides to form 1,4-disubsituted-1,2,3-triazoles.
      Please see the link below to review an introduction to chemistry:
      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/chemistry-and-synthesis/adc-and-bioconjugation/click-chemistry

      Please see the link below to review product 777358, Cy5-alkyne:
      https://www.sigmaaldrich.com/product/aldrich/777358

      A search of the scientific literature may be carried out to develop the intended application. The suggested key terms of interest may be azide, alkyne, and click chemistry.

      Helpful?

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