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777323

Sigma-Aldrich

Cy5-azide

95% (HPLC)

Synonim(y):

Sonda fluorescencyjna azydku Cy5

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1 MG
696,00 zł
5 MG
1930,00 zł

696,00 zł

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1 MG
696,00 zł
5 MG
1930,00 zł

About This Item

Wzór empiryczny (zapis Hilla):
C49H78N8O10S3
Masa cząsteczkowa:
1035.39
Kod UNSPSC:
12352125
Identyfikator substancji w PubChem:
329767899
NACRES:
NA.22

696,00 zł

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Poproś o zamówienie zbiorcze

Poziom jakości

100

Próba

95% (HPLC)

Formularz

solid

przydatność reakcji

reaction type: click chemistry

ε (extinction coefficient)

251,000

fluorescencja

λex 647 nm; λem 663 nm

temp. przechowywania

−20°C

ciąg SMILES

CC1(C)C(/C=C/C=C/C=C(N2CCCS([O-])(=O)=O)\C(C)(C)C3=C2C=CC(S([O-])(=O)=O)=C3)=[N+](CCCCCC(NCCCN=[N+]=[N-])=O)C4=C1C=C(S([O-])(=O)=O)C=C4.CC[NH+](CC)CC.CC[NH+](CC)CC

InChI

1S/C37H48N6O10S3.2C6H15N/c1-36(2)29-25-27(55(48,49)50)16-18-31(29)42(22-10-6-9-15-35(44)39-20-11-21-40-41-38)33(36)13-7-5-8-14-34-37(3,4)30-26-28(56(51,52)53)17-19-32(30)43(34)23-12-24-54(45,46)47;2*1-4-7(5-2)6-3/h5,7-8,13-14,16-19,25-26H,6,9-12,15,20-24H2,1-4H3,(H3-,39,44,45,46,47,48,49,50,51,52,53);2*4-6H2,1-3H3

Klucz InChI

NJSWGOLBDDXJQO-UHFFFAOYSA-N

Powiązane kategorie

Zastosowanie

Azide modified cyanine dye that can be used to detect or label alkyne- or cyclooctyne-containing molecules or biomolecules by fluorescence spectroscopy following azide-alkyne cycloaddition.

Solubility: DMSO, DMF, MeOH
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Piktogramy

Exclamation mark
GHS07

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H315,H319,H335

Zwroty wskazujące środki ostrożności

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Klasyfikacja zagrożeń

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Organy docelowe

Respiratory system

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
MKCW7635
MKCV9501
MKCR9311
MKCQ7519
MKCR4683

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Jeśli potrzebujesz konkretnej wersji, możesz wyszukać konkretny certyfikat według numeru partii lub serii.

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Produkty

Click Chemistry in Drug Discovery

Explore the principles and applications of click chemistry in drug discovery, highlighting efficient reactions that streamline the synthesis of bioactive compounds.

Fluorescence Lifetime Measurement

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

Pomiar czasu życia fluorescencji

Pomiar czasu życia fluorescencji ma przewagę nad pomiarami opartymi na intensywności. Zastosowania obejmują testy czasu życia fluorescencji, wykrywanie i FLI.

Questions

1–3 of 3 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Hi, I am trying to conjugate cy5 to a protein already has azide at its C-terminus. What would the protocol would be in this case? Do we need to use CuAAC in our case?

    1 answer

    1. This product is a Cy5-azide. Conjugation may not be possible if the protein of interest has a terminal azide rather than a terminal alkyne. Should this be the case, a Cy5-alkyne such as product 777358, would be recommended. Click chemistry relies on 1,3-dipolar cycloaddition of alkynes to azides to form 1,4-disubsituted-1,2,3-triazoles.
      Please see the link below to review an introduction to chemistry:
      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/chemistry-and-synthesis/adc-and-bioconjugation/click-chemistry

      Please see the link below to review product 777358, Cy5-alkyne:
      https://www.sigmaaldrich.com/product/aldrich/777358

      A search of the scientific literature may be carried out to develop the intended application. The suggested key terms of interest may be azide, alkyne, and click chemistry.

      Helpful?

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