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Merck

SRP3138

Sigma-Aldrich

PDGF-BB human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

别名:

Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF), Platelet-Derived Growth Factor-BB

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About This Item

分類程式碼代碼:
12352202
eCl@ss:
32190102
NACRES:
NA.32

生物源

human

重組細胞

expressed in E. coli

化驗

≥98% (HPLC)
≥98% (SDS-PAGE)

形狀

lyophilized

效力

1.0-3.0 ng/mL ED50

分子量

24.3 kDa

包裝

pkg of 10 μg

技術

cell culture | mammalian: suitable

雜質

<0.1 EU/μg endotoxin, tested

顏色

white to off-white

UniProt登錄號

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

human ... PDGFB(5155)

一般說明

PDGFs (platelet-derived growth factors) are disulfide-linked dimers consisting of two 12.0-13.5kDa polypeptide chains, designated PDGF-A and PDGF-B chains. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. The gene PDGF-B is mapped to human chromosome 22q13.1. Recombinant human PDGF-BB is a 24.3kDa disulfide-linked homodimer of two B chains (218 total amino acids).

應用

PDGF-BB human has been used in the cell viability assay. It has been used as a stimulator in PDGFRα (platelet-derived growth factor receptor α)-positive HTPCs (human testicular peritubular cells).

生化/生理作用

The three naturally occurring PDGFs (platelet-derived growth factors); PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. PDGF-B along with PDGFR-β participates in vascular maturation. It brings smooth muscle cells and pericytes to growing vessels. Heterozygous mutation in PDGF-B is associated with primary familial brain calcification.

序列

SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT

外觀

Lyophilized from 10 mM Acetic Acid.

重構

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Human testicular peritubular cells host putative stem Leydig cells with steroidogenic capacity.
Landreh L, et al.
The Journal of Clinical Endocrinology and Metabolism, 99, E1227-E1235 (2014)
Dovitinib preferentially targets endothelial cells rather than cancer cells for the inhibition of hepatocellular carcinoma growth and metastasis.
Chen ZY, et al.
Journal of Translational Medicine, 10, 245-245 (2012)
Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty.
Nili N, et al.
The American Journal of Pathology, 163, 869-878 (2003)
Roles of PDGF in animal development.
Hoch RV and Soriano P
Development, 130, 4769-4784 (2003)
Platelets in inflammation and atherogenesis.
Gawaz M, et al.
The Journal of Clinical Investigation, 115, 3378-3384 (2005)

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