推荐产品
生物源
human
品質等級
化驗
>98% (SDS-PAGE)
製造商／商標名
Chemicon®
技術
cell culture | mammalian: suitable
雜質
<0.1 ng/μg endotoxin (of PDGF-BB; 1EU/μg)
輸入
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
UniProt登錄號
運輸包裝
dry ice
基因資訊
human ... PDGFB(5155)
一般說明
产品来源：大肠杆菌
分离自血小板的生长因子（PDGF）是一种有效的促细胞分裂剂，适用于多种细胞类型，包括成纤维细胞、平滑肌和结缔组织。由称为A链和B链形成的二聚体组成了PDGF，其可以AA或BB同二聚体或AB异二聚体形式存在。重组人PDGF-BB是由两条B链（共218个氨基酸）组成的24.3 kDa二硫键连接的同型二聚体。
應用
分离自血小板的生长因子（PDGF）是一种有效的促细胞分裂剂，适用于多种细胞类型，包括成纤维细胞、平滑肌 & 结缔组织。
对于大多数体外应用，PDGF-BB在0.2至10.0 ng/mL的浓度范围内发挥其生物学活性。响应的细胞包括（部分列表）：成纤维细胞、结缔组织。
聯結
替代品：GF018
外觀
从10 mM HOAc中冻干的无菌过滤溶液。
无菌的过滤溶液是从10mM HOAc中冻干得到。
儲存和穩定性
冻干的PDGF-BB在室温下可稳定保存数周，但最好在-20°C下保存。快速离心后，在水中重溶至0.1-1.0 mg/mL的浓度。该溶液可以在缓冲水溶液中进行稀释，并可在2-8°C中保存一周或在-20°C保存供将来使用。重溶的PDGF-BB应以未稀释的等分形式保存于-20°C中，可稳定保存长达六个月。
分析報告
比活度：与标准品相比，人PDGF-BB具有完全的生物活性。通过其对BALB/c 3T3细胞的剂量依赖性有丝分裂活性所确定的ED50为6-12 ng/mL。
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
其他客户在看
Karla Lucia F Alvarez et al.
STAR protocols, 3(1), 101149-101149 (2022-02-11)
Here, we present a protocol to culture primary human vascular smooth muscle cells (VSMCs) under Alzheimer's disease (AD)-like conditions, including steps for morphological characterization with microscopy. We then describe functional assays, including wound healing, transwell, coculture, and supernatant assays, to
Yi Ma et al.
Journal of cellular and molecular medicine, 25(11), 5202-5219 (2021-05-05)
Pulmonary arterial hypertension (PAH) featured a debilitating progressive disorder. Here, we intend to determine diagnosis-valuable biomarkers for PAH and decode the fundamental mechanisms of the biological function of these markers. Two mRNA microarray profiles (GSE70456 and GSE117261) and two microRNA
Xunde Xian et al.
eLife, 6 (2017-11-17)
Low-density lipoprotein receptor-related protein 1 (LRP1) is a multifunctional cell surface receptor with diverse physiological roles, ranging from cellular uptake of lipoproteins and other cargo by endocytosis to sensor of the extracellular environment and integrator of a wide range of
Lifeng Jiang et al.
Frontiers in medicine, 9, 886868-886868 (2022-07-16)
Hyper-proliferation of pulmonary arterial vascular smooth muscle cells (PAVSMC) is an important pathological component of pulmonary vascular remodeling in pulmonary arterial hypertension (PAH). Lipogenesis is linked to numerous proliferative diseases, but its role in PAVSMC proliferation in PAH remains to
Zili Zhang et al.
BioFactors (Oxford, England), 47(5), 801-818 (2021-06-16)
Targeting the elimination of activated hepatic stellate cells (HSCs) and blocking excessive deposition of extracellular matrix are recognized as an effective strategy for the treatment of hepatic fibrosis. As a newly discovered programmed cell death mode, the regulatory mechanism of
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门