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Merck
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H5791

Sigma-Aldrich

肝细胞生长因子 人

HGF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

别名:

HGF, 散射因子, 肝素A

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About This Item

MDL號碼:
MFCD00217422
分類程式碼代碼:
12352202
NACRES:
NA.77

生物源

human

品質等級

100

重組細胞

expressed in HEK 293 cells

化驗

≥95% (SDS-PAGE)

形狀

lyophilized powder

效力

≤20 ng/mL EC50

品質

endotoxin tested

分子量

monomer 70 kDa (glycosylated)

包裝

pkg of 5x10 μg
pkg of 10 μg

儲存條件

avoid repeated freeze/thaw cycles

技術

cell culture | mammalian: suitable

雜質

≤1 EU/mg

UniProt登錄號

P14210

儲存溫度

−20°C

基因資訊

human ... HGF(3082)

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一般說明

The HGF gene spans ∼70 kb and consists of 18 exons interrupted by 17 introns. The organization of the human HGF gene is highly homologous to that of human plasminogen. HGF maps to the long arm of human chromosome 7, 7q21.1.

生化／生理作用

HGF is a multifunctional cytokine that promotes mitogenesis, migration, invasion, and morphogenesis. It is a potent mitogen, stimulating the growth of hepatocytes, renal tubular epithelial cells, epidermal keratinocytes, epidermal melanocytes, Mv1Lu (mink lung epithelial cells), and BALB/MK (mouse keratinocytes). HGF inhibits the growth of B6/F1 (mouse melanoma) cells, KB (human squamous carcinoma) cells, and HepG2 (human hepatoma) cells. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.
肝细胞生长因子（HGF）是肝细胞和多种细胞类型（包括内皮和上皮细胞、黑素细胞和角质形成细胞）的有效促分裂原。它还可介导上皮形态发生。HGF是由met原癌基因编码的酪氨酸激酶受体的配体。

其他說明

NSO表达的重组HGF含有大于95％的异二聚体HGF。昆虫细胞系表达的重组HGF是单链HGF（主要）和一些异二聚体HGF的混合物。单链HGF和异二聚体HGF在 体外 分析中具有相同的活性。

外觀

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

準備報告

HumanKine HGF is expressed as a glycosylated 70 kDa single chain monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

分析報告

The specific activity was determined by the dose-dependent stimulation of the proliferation of the monkey epithelial cell line 4MBr-5.

法律資訊

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Karène Mahtouk et al.
Biochimica et biophysica acta, 1806(2), 208-219 (2010-07-27)
Hepatocyte growth factor (HGF) and its receptor MET are essential during embryonic development and throughout postnatal life. However, aberrant MET activation, due to overexpression, mutations, or autocrine ligand production, contributes to the development and progression of a variety of human
T Nakamura et al.
Nature, 342(6248), 440-443 (1989-11-23)
Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. The partial purification
Rajani Kanteti et al.
PloS one, 9(9), e105919-e105919 (2014-09-16)
Malignant pleural mesothelioma (MPM) is an aggressive disease with a poor prognosis. Studies have shown that both MET and its key downstream intracellular signaling partners, PI3K and mTOR, are overexpressed in MPM. Here we determined the combinatorial therapeutic efficacy of
Frieda Kage et al.
Journal of cell science, 135(15) (2022-08-17)
Cell migration frequently involves the formation of lamellipodia induced by Rac GTPases activating WAVE regulatory complex (WRC) to drive Arp2/3 complex-dependent actin assembly. Previous genome editing studies in B16-F1 melanoma cells solidified the view of an essential, linear pathway employing
Hiroshi Kusunoki et al.
Hypertension research : official journal of the Japanese Society of Hypertension, 37(6), 500-506 (2014-03-14)
The paracrine loop involving inflammatory cytokines between adipocytes and macrophages establishes a vicious cycle that aggravates pro-inflammatory changes in adipose tissue. The serum level of hepatocyte growth factor (HGF) is increased in metabolic syndrome, but whether HGF is beneficial or
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