推荐产品
生物来源
mouse
质量水平
偶联物
peroxidase conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
1A4, monoclonal
表单
lyophilized powder
分子量
antigen ~42 kDa
种属反应性
frog, mouse, hamster, chicken, viper, bovine, crayfish, goat, rat, cat, sheep, guinea pig, lizard, human, rabbit, dog, snail
技术
direct ELISA: suitable
immunoblotting: 2-4 μg/mL using extracts of mouse heart tissues
immunohistochemistry: 5-10 μg/mL using human appendix or tonsil tissue sections
同位素/亚型
IgG2a
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... ACTA2(59)
一般描述
Monoclonal Anti-Actin, α-Smooth Muscle-HRP (mouse IgG2a isotype) is derived from the hybridoma 1A4 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a synthetic peptide corresponding to a sequence of α-smooth muscle actin, conjugated to KLH. Actin is a highly conserved protein, expressed as six isoforms and represents differentiation markers of muscle tissues.
免疫原
a synthetic peptide corresponding to a sequence at the NH2 terminal synthetic decapeptide of α-smooth muscle actin, conjugated to KLH.
应用
Monoclonal Anti-Actin, α-Smooth Muscle - Peroxidase antibody has been used in:
- direct-immunoblotting
- direct-immunohistochemistry
- direct- enzyme linked immunosorbent assay (ELISA)
- direct staining of tissues and cells
- characterization of stromal cell heterogeneity in various organs and distinguish smooth muscle cells from fibroblasts in mixed cultures.
生化/生理作用
Actin and Myosin are two major cytoskeletal proteins implicated in cell motility, both constitutively expressed in many cells types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. It has been shown that relative proportions of actin isoforms are diverse in smooth muscles of different organs and change within the same population of smooth muscle cells during development, pathological situations and different culture conditions.
外形
Supplied as lyophilized powder. After reconstitution with 0.1 mL of distilled water to a final antibody concentration of ∼ 2 mg/mL, the solution contains 1% BSA, 2.5% trehalose, 0.05% MIT in 0.01 M sodium phosphate buffered saline
制备说明
Reconstitute in 0.1mL of distilled water.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Danger
危险分类
Aquatic Chronic 3 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
其他客户在看
A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation.
Skalli O, et al.
The Journal of Cell Biology, 103(6), 2787-2796 (1986)
Ayelén M Santamans et al.
Science advances, 10(3), eadk6524-eadk6524 (2024-01-19)
Pulmonary hypertension (PH) can affect both pulmonary arterial tree and cardiac function, often leading to right heart failure and death. Despite the urgency, the lack of understanding has limited the development of effective cardiac therapeutic strategies. Our research reveals that
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