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Merck
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C6198

Sigma-Aldrich

抗-肌动蛋白, α-平滑肌- Cy3™抗体，小鼠单克隆

clone 1A4, purified from hybridoma cell culture

别名:

单克隆抗-肌动蛋白，α-平滑肌, α-平滑肌肌动蛋白- Cy3™抗体, α-肌动蛋白2 Cy3, SMA

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.43

生物源

mouse

共軛

CY3 conjugate

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

1A4, monoclonal

形狀

buffered aqueous solution

分子量

antigen ~42 kDa

物種活性

human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake

儲存條件

protect from light

技術

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tonsil or appendix

同型

IgG2a

UniProt登錄號

P62736

應用

research pathology

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

基因資訊

human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)

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相关类别

一般說明

其抗体（也称为抗α-Sm-1）对α-平滑肌肌动蛋白的单一异构体具有特异性。它在免疫印迹试验中可与α-平滑肌肌动蛋白发生特异性反应，并可在冷冻或福尔马林固定的、或石蜡包埋的组织切片中对平滑肌细胞进行标记。
单克隆抗肌动蛋白α-平滑肌（小鼠IgG2a同种型）源自小鼠骨髓瘤细胞和免疫BALB/c小鼠脾细胞融合产生的1A4杂交瘤

免疫原

N-末端合成的α-平滑肌肌动蛋白十肽。

應用

Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
使用单克隆抗ACTA2抗体对来自牛主动脉的平滑肌细胞进行免疫细胞化学分析。首先将细胞在玻璃盖玻片上生长，并在4％的50％丙酮/乙醇中固定10分钟。
这一抗体成功运用案例，以及相关综述文献，如下：
流式细胞仪/细胞分选（2篇文献）
免疫组织化学（3篇）
免疫荧光（13篇）
免疫组织化学（27篇）
Western印迹法（2篇）

生化／生理作用

在鸡和大鼠胚胎成肌过程中，平滑肌肌动蛋白可与肌节α-肌动蛋白进行瞬时共表达。在成年哺乳动物心脏的心室传导通道中，经证存在这种物质。它在平滑肌瘤、平滑肌肉瘤和平滑肌母细胞瘤以及部分横纹肌肉瘤中表达。

外觀

溶解保存在0.01M磷酸缓冲盐,pH7.4, 含有1%牛血清白蛋白和15 mM叠氮化钠。

其他說明

想要查看肌动蛋白抗体选择指南，请访问 www.sigmaaldrich.com/actin。

法律資訊

Cy在 Amersham Biosciences Limited许可下销售。
Cy3 is a trademark of Cytiva

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Coexpression of alpha-sarcomeric actin, alpha-smooth muscle actin and desmin during myogenesis in rat and mouse embryos I. Skeletal muscle
Babai F, et al.
Differentiation, 44(2), 132-142 (1990)
Filipa Moraes et al.
Circulation research, 113(9), 1076-1086 (2013-07-31)
Arteriogenesis is the process of formation of arterial conduits. Its promotion is an attractive therapeutic strategy in occlusive atherosclerotic diseases. Despite the functional and clinical importance of arteriogenesis, the biology of the process is poorly understood. Synectin, a gene previously
Steven L Fabian et al.
The American journal of pathology, 180(4), 1441-1453 (2012-02-22)
The Hedgehog (Hh) signaling pathway regulates tissue patterning during development, including patterning and growth of limbs and face, but whether Hh signaling plays a role in adult kidney remains undefined. In this study, using a panel of hedgehog-reporter mice, we
Rebeca Ortega et al.
Arteriosclerosis, thrombosis, and vascular biology, 39(8), 1614-1628 (2019-07-12)
Abdominal aortic aneurysm (AAA) is a pathological condition of permanent vessel dilatation that predisposes to the potentially fatal consequence of aortic rupture. SGLT-2 (sodium-glucose cotransporter 2) inhibitors have emerged as powerful pharmacological tools for type 2 diabetes mellitus treatment. Beyond
Jing Huang et al.
World journal of cardiology, 7(10), 671-684 (2015-10-31)
To study the genesis of neointima formation in pulmonary hypertension (PH), we investigated the role of caveolin-1 and related proteins. Male Sprague Dawley rats were given monocrotaline (M, 40 mg/kg) or subjected to hypobaric hypoxia (H) to induce PH. Another
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