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MITOISO1

Sigma-Aldrich

线粒体分离试剂盒

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

别名:

Mitochondria Purification Kit

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About This Item

EC 号:
232-650-8
UNSPSC代码:
12352200
NACRES:
NA.32

质量水平

200

用途

sufficient for 10-20 g (animal tissue)
 sufficient for 50 assays (2 mL)

保质期

2 yr

技术

fractionation: suitable

运输

dry ice

储存温度

−20°C

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应用

线粒体分离试剂盒可简单、快速地分离动物组织中富集的线粒体组分，并检测线粒体内膜的电化学质子梯度(ΔΨ)。用于线粒体介导的细胞凋亡研究。
线粒体分离试剂盒已用于：
  • 分离线粒体和胞浆组分，用于测定支链氨基酸转氨酶(BCAT)活性
  • 分离肾小球和心脏线粒体
  • 分离脑损伤样品中的线粒体，检测线粒体跨膜电位

特点和优势

  • 特制提取剂和可靠实验方案适合少量分离——在离心管中即可分离富集完整线粒体组分。
  • 获得完整有活性的线粒体——所得线粒体适用于细胞凋亡、氧化应激等体外分析研究。
  • 提供可确定内膜完整性的染色剂和实验方案——无需购买其他试剂，即可确定线粒体内膜完整性。
  • 适配细胞色素C氧化酶检测试剂盒——可轻松确定线粒体外膜完整性。

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • T9201Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solid化学品安全说明书

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象形图

Health hazardExclamation mark
GHS08,GHS07

警示用语：

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

10 - Combustible liquids

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000284757
0000222575
11171134
118M4156V
087M4135V

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Cancer research, 65(8), 3162-3170 (2005-04-19)
5-Fluorouracil (5-FU) is widely used for treatment of advanced colorectal cancer. However, it is common for such patients to develop resistance to 5-FU, and this drug resistance becomes a critical problem for chemotherapy. The mechanisms underlying this resistance are largely
Mitochondrial dysfunction mediates aldosterone-induced podocyte damage: a therapeutic target of PPARgamma
Zhu C, et al.
The American Journal of Pathology, 178(5), 2020-2031 (2011)
Neuroprotective effect of hyperbaric oxygen therapy in brain injury is mediated by preservation of mitochondrial membrane properties
Palzur E, et al.
Brain Research, 1221, 126-133 (2008)
Jacinta Serpa et al.
The Journal of biological chemistry, 285(50), 39211-39223 (2010-10-12)
The short chain fatty acid (SCFA) butyrate is a product of colonic fermentation of dietary fibers. It is the main source of energy for normal colonocytes, but cannot be metabolized by most tumor cells. Butyrate also functions as a histone
Diederik Moechars et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 26(46), 12055-12066 (2006-11-17)
Uptake of L-glutamate into synaptic vesicles is mediated by vesicular glutamate transporters (VGLUTs). Three transporters (VGLUT1-VGLUT3) are expressed in the mammalian CNS, with partial overlapping expression patterns, and VGLUT2 is the most abundantly expressed paralog in the thalamus, midbrain, and
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